20.500.12556/DiRROS-25468 Early stress detection in forest trees using a nanobody-functionalized electrochemical biosensor for ascorbate peroxidase Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature such as heavy metal contamination, drought, or pest infestations which may lead to their massive decline. We designed a comprehensive approach for isolating, producing and functionalizing reagents suitable for the affordable detection of forest plant stress biomarkers with the aim to provide quantitative data to assess plant stress fluctuation and, possibly, to design mitigation strategies. We first optimized a panning protocol to recover nanobodies targeting shared sequences that could cross-react with both Pisum sativum and Populus nigra ascorbate peroxidase (APX). After their production as recombinant constructs and their extensive biophysical and biochemical characterization, such reagents were exploited as the immunocapture element of an electrochemical biosensor conceived as a potential point-of-care device. Such biosensor could detect both pea and poplar APX in leaf extracts and could be used to clearly discriminate between control and heavy metal-stressed poplar plants based on their APX activity, even before the appearance of any phenotypic symptom. The combination of fast and inexpensive reagent production with the development of portable diagnostics opens the opportunity for large-scale, on-site surveys of forest trees. plant stress scavengers diagnostics nanobodies biosensors true false true Angleški jezik Ni določen Neznano 2026-01-21 10:53:18 2026-01-21 10:53:19 2026-01-23 03:42:11 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 11. 4. 2025; 8 str. [article no.] ǂ100844 Vol. 16 Jun. 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 577 2667-064X 10.1016/j.stress.2025.100844 232353027 https://www.sciencedirect.com/science/article/pii/S2667064X25001095?via%3Dihub 1 0574954e-f6af-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=38594 1-s2.0-S2667064X25001095-main.pdf 1-s2.0-S2667064X25001095-main.pdf 1 BC95E4EC8FF3CB80C39E101971F7204A 4c3636a8043328205302324d6879dfa80de655205752fe7fa2e3005422cc5fc9 5b607465-f6af-11f0-a15d-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=38597 Gozdarski inštitut Slovenije 0 0 0