20.500.12556/DiRROS-24784 Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal region Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY. potato virus Y potato coat proteins viral movement point mutations krompirjev virus Y krompir plaščni proteini gibanje virusa točkovne mutacije true false true Angleški jezik Slovenski jezik Neznano 2025-12-18 11:14:00 2025-12-18 11:14:01 2026-01-09 03:52:10 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll, Tjaša Lukan; Članek v PDF formatu obsega 14 str.; Opis vira z dne 2. 12. 2025; Online first: 20. Nov. 2025; iss. 12 Vol. 99 Dec. 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 2025-12-23 577.2 1098-5514 10.1128/jvi.01400-25 259503619 https://doi.org/10.1128/jvi.01400-25 1 51c854a0-dbfa-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=37055 jvi.01400-25-s0001.pdf jvi.01400-25-s0001.pdf 1 9833B6CED5E00BB97DE13554EE3888EA 2ed0100157b368f34f8cb41aeaa4b343e8ba88d96bb426a8289b86c7deea21cd db2bfaac-ec82-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=37586 RAZ_Vozelj_Anze_2025.pdf RAZ_Vozelj_Anze_2025.pdf 1 A20E2ABF5299B82BC4945B7A4B66D223 436e5e0e7fdcbd4965390e862133f9e3eda0a1265978e40303f0f6b1878577ee f28fd787-ec82-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=37587 Nacionalni inštitut za biologijo Metadata from: Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal region 0 0 0