20.500.12556/DiRROS-24697 Prolonged hospitalization signature and early antibiotic effects on the nasopharyngeal resistome in preterm infants Respiratory pathogens, commonly colonizing nasopharynx, are among the leading causes of death due to antimicrobial resistance. Yet, antibiotic resistance determinants within nasopharyngeal microbial communities remain poorly understood. In this prospective cohort study, we investigate the nasopharynx resistome development in preterm infants, assess early antibiotic impact on its trajectory, and explore its association with clinical covariates using shotgun metagenomics. Our findings reveal widespread nasopharyngeal carriage of antibiotic resistance genes (ARGs) with resistomes undergoing transient changes, including increased ARG diversity, abundance, and composition alterations due to early antibiotic exposure. ARGs associated with the critical nosocomial pathogen Serratia marcescens persist up to 8–10 months of age, representing a long-lasting hospitalization signature. The nasopharyngeal resistome strongly correlates with microbiome composition, with inter-individual differences and postnatal age explaining most of the variation. Our report on the collateral effects of antibiotics and prolonged hospitalization underscores the urgency of further studies focused on this relatively unexplored reservoir of pathogens and ARGs. preterm infants respiratory resistome whole genome sequencing genetics drug effects true false true Angleški jezik Ni določen Neznano 2025-12-12 14:11:32 2025-12-12 14:11:33 2025-12-13 03:51:10 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 10. 11. 2025; str. 1-13 article no. 6024 Vol. 15 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61:575 2041-1723 10.1038/s41467-024-50433-7 256494851 RAZ_Dhariwal_Achal_2024.pdf RAZ_Dhariwal_Achal_2024.pdf 1 BC621742F8CF996A682F4C12481EF732 872c67f8fd100ebf92c411f6885940de922484706de62a8e63868d9498299610 55a68049-d75c-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36880 https://www.nature.com/articles/s41467-024-50433-7 0 624eac8f-d75c-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36881 Univerzitetni klinični center Ljubljana 0 0 0