20.500.12556/DiRROS-24674 L-FABP as a potential biomolecular marker of liver graft injury Background: In recent years, indications for liver transplantation have expanded, while the age of transplant recipients has significantly increased due to improvements in perioperative management. As clinical manifestations of posttransplant complications vary and are often nonspecific, the identification of appropriate biomarkers is important for the assessment of graft quality and early recognition of potential complications following liver transplantation. Liver-type FABP (L-FABP) is a small cytoplasmic protein found abundantly in hepatocytes and is involved in the intracellular transport of long-chain fatty acids. Elevated serum levels have been detected in acute and chronic liver failure, kidney failure, and some malignancies. Materials and Methods: We conducted a prospective, single-center study from July 2023 to January 2025, including 29 adult patients who underwent deceased-donor transplantation. Three patients were excluded due to inadequate sample withdrawals. Serum L-FABP was measured preoperatively and on postoperative days 1, 3, 5, 7, and 14. Clinical, surgical, and biochemical data were collected and analyzed using non-parametric statistical tests. Results: L-FABP levels were significantly higher on POD 7 in recipients of grafts from donors ≥ 65 years (p = 0.035), with no corresponding changes in standard liver function markers. While no significant differences in L-FABP levels were found between patients with and without infectious biliary or vascular complications (all p > 0.05), we proved a strong negative correlation between intraoperative blood transfusion volume and L-FABP levels on POD 5 (ρ = −0.677, p < 0.001) and POD 7 (ρ = −0.455, p = 0.025). Conclusions: Our findings suggest that L-FABP holds promise as a biomarker for the early detection of subclinical hepatic graft cellular injury, which is not detected by means of conventional biomarkers for liver function. liver transplantation biomarker L-FABP hepatic graft injury marginal liver graft true false true Angleški jezik Ni določen Neznano 2025-12-11 14:20:43 2025-12-11 14:20:43 2025-12-12 03:44:12 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 3. 11. 2025; str. 1-16 iss. 20, [article no.] 7404 Vol. 14 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 616.61 2077-0383 10.3390/jcm14207404 255639811 RAZ_Kalamutova_Ana_2025.pdf RAZ_Kalamutova_Ana_2025.pdf 1 B20281424F3216F72CE3C8CB938FA18A 881f0336b03da79da047a8b6b1e6d8f321efe65ed2f9b58491a824250df0d1f7 71d1d3c5-d694-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36838 https://www.mdpi.com/2077-0383/14/20/7404 0 83c13f99-d694-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36839 Univerzitetni klinični center Ljubljana 0 0 0