20.500.12556/DiRROS-24507 MicroRNA expression profiles in sinonasal biopsies to support diagnosis of granulomatosis with polyangiitis Objectives: To identify aberrantly expressed microRNAs (miRNAs) in sinonasal tissue biopsies of patients with granulomatosis with polyangiitis (GPA), associate their expression profiles to sinonasal histopathology, and assess their differential expression between subgroups of clinically proven GPA patients, healthy controls and patients exhibiting inflammation of other etiology. Methods: We included formalin-fixed, paraffin-embedded biopsy tissue samples of sinonasal mucosa from 37 patients with clinically proven GPA, 15 patients with inflammation of other etiology and 14 control patients with normal histology. Of the included GPA patients, 20 patients had characteristic GPA-related histological features, while 17 patients displayed non-specific GPA histopathology in their sinonasal biopsy. Assessment of histological parameters was performed using histopathological techniques, and analysis of miRNA expression with miRCURY LNA miRNA miRNome Human PCR Panels and quantitative real-time PCR. Results: We determined expression of 306 miRNAs in sinonasal biopsy samples, which displayed different extent of dysregulation between individual patient groups. Based on their potential to discriminate between the controls, non-GPA and GPA patient subgroups, dysregulation of 11 miRNAs was further assessed, of which miR-1-3p/-21-3p/-93-5p/-155-5p/-1248/-31-3p/-182-5p/-183-5p and let-7b-5p held the potential to stratify patients based on their sinonasal tissue miRNA profile. Notably, several of these miRNAs were associated with the presence of granulomas, vasculitis and necrosis in sinonasal biopsies of GPA patients. Conclusion: Our study identifies novel miRNAs putatively implicated in the pathogenesis of GPA, and highlights dysregulated miRNAs as supporting biomarkers in establishing GPA diagnosis, particularly in the early phases of the disease, or in patients with atypical GPA presentation. biomarker granulomatosis with polyangiitis inflammation microRNA sinonasal tissue biološki označevalec granulomatoza s poliangiitisom vnetje mikroRNA sinonazalno tkivo true false true Angleški jezik Slovenski jezik Neznano 2025-12-03 13:12:17 2025-12-03 13:12:17 2025-12-04 03:46:30 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 30. 4. 2025; str. 1-14 Vol. 16 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 616-002 1664-3224 10.3389/fimmu.2025.1579750 234656003 RAZ_Zivanovic_Milanka_2025.pdf RAZ_Zivanovic_Milanka_2025.pdf 1 DD1554587C6335DCFAEBA7B8FAA53FAD 19aaad87e02eb356109b214ae6e0f1b66e5864509ddcbb926b5cc79b22341e7e 6dc946c7-d041-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36531 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1579750/full 1 51b5bdb2-d041-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36528 Univerzitetni klinični center Ljubljana 0 0 0