20.500.12556/DiRROS-24479 Fecal short-chain fatty acids are associated with obesity in gestational diabetes Background: Short-chain fatty acids (SCFAs), which are produced by the microbial fermentation of undigested carbohydrates, play an important role in the metabolism and physiology of the host. SCFAs are involved in the regulation of maternal metabolism during pregnancy and influence weight gain, glucose metabolism, and metabolic hormones. Methods: In 2017, women who were treated for gestational diabetes mellitus (GDM) at the University Medical Centre Ljubljana were invited to participate in a longitudinal study. A total of 45 women were included in this study and comprehensively phenotyped. During the second and third trimester of pregnancy, the women with GDM provided fecal samples for SCFA analysis. The samples were analyzed by high-performance liquid chromatography for the simultaneous determination of acetate, propionate, and butyrate. Results: SCFA concentrations in feces differed between overweight/obese and normal-weight women with GDM. Acetate and propionate concentrations were significantly higher in pregnant women who were overweight or obese before pregnancy compared to normal-weight women but butyrate concentrations were not. Butyrate was elevated in the third trimester in the group with excessive gestational weight gain. Conclusions: The relationship between SCFAs and obesity is complex, and the association between SCFAs and GDM remains to be clarified. Regardless of the conflicting publications on the role of SCFAs, our study showed that higher acetate and propionate levels were associated with the weight categories of overweight or obesity before pregnancy and higher butyrate levels were associated with excessive gestational weight gain. fecal SCFA pregnancy gestational diabetes mellitus preconception BMI gestational weight gain črevesne kratkoverižne maščobne kisline nosečnost sladkorna bolezen indeks telesne teže true false true Angleški jezik Slovenski jezik Neznano 2025-12-01 13:52:13 2025-12-01 13:52:13 2025-12-02 03:42:03 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 12. 2. 2025; str. 1-13 iss. 2, [article no.] 387 Vol. 13 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 618.2:613.2 2227-9059 10.3390/biomedicines13020387 225946115 RAZ_Molan_Katja_2025.pdf RAZ_Molan_Katja_2025.pdf 1 D66D0078A9B33BF3B67AA63F98301408 a0847c6a714251ba662305be6a4a73117223ed11badd18b9db6cd1e4742306b4 b07feae5-ceb4-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36475 https://www.mdpi.com/2227-9059/13/2/387 1 90909a49-ceb4-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36474 Univerzitetni klinični center Ljubljana 0 0 0