20.500.12556/DiRROS-24475 Human primary lens epithelial cultures on basal laminas studied by synchrotron-based FTIR microspectroscopy for understanding posterior capsular opacification Human primary lens epithelial cultures serve as an in vitro model for posterior capsular opacification (PCO) formation. PCO occurs when residual lens epithelial cells (LECs) migrate and proliferate after cataract surgery, differentiating into fibroblastic and lens fiber-like cells. This study aims to show and compare the bio-macromolecular profiles of primary LEC cultures and postoperative lens epithelia LECs on basal laminas (bls), while also analyzing bls and cultured LECs separately. Using synchrotron radiation-based Fourier transform infrared (SR-FTIR) (Bruker, Karlsruhe, Germany) microspectroscopy at the Spanish synchrotron light source ALBA, we observed that the SR-FTIR measurements were predominantly influenced by the strong collagen absorbance of the bls. Cultured LECs on bls showed a higher collagen contribution, indicated by higher vas CH3 , CH2 and CH3 wagging and deformation, and the C–N stretching of collagen. In contrast, postoperative LECs on bls showed a higher cell contribution, indicated by the vsym CH2 peak and the ratio between vas CH2 and vas CH3 peaks. The primary difference revealed using SR-FTIR is the greater LEC contribution in spectra recorded from postoperative lens epithelia compared to cultured LECs on bls. IR spectra for bl, cultured LECs and postoperative lens epithelia could be valuable for future research primary lens culture human lens capsule lens epithelial cells synchrotron light true false true Angleški jezik Ni določen Neznano 2025-12-01 13:04:55 2025-12-01 13:04:55 2025-12-02 03:42:02 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Opis vira z dne 22. 1. 2025; str. 1-16 issue 16, [article no. 8858] Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 61 1422-0067 10.3390/ijms25168858 223412739 RAZ_Andjelic_Sofija_2024.pdf RAZ_Andjelic_Sofija_2024.pdf 1 C331C0B31A425B6FF463341B34DFD05F 72f29f5b35e397ed0d1c58f39b09bf3a74bf8a7a47a55b73df48ff5b3f651ac9 0b8a730f-ceae-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36467 https://www.mdpi.com/1422-0067/25/16/8858 1 f4aafe44-cead-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36466 Univerzitetni klinični center Ljubljana 0 0 0