20.500.12556/DiRROS-24459 Adjuvant treatment with empagliflozin or semaglutide increases endothelial progenitor cells in subjects with well-controlled type 1 diabetes mellitus Circulating endothelial cells (CECs) and endothelial progenitor cells (EPCs) are promising markers of vascular damage and endothelial regeneration potential. We focused on the detection of CECs and EPCs using flow cytometry with regard to analytical challenges and its suitability for routine testing. As part of a clinical validation, CECs and EPCs were measured in blood samples from 83 subjects with type 1 diabetes (T1DM), evaluating an adjuvant intervention with two different antidiabetic drugs, empagliflozin (N = 28) and semaglutide (N = 29). Both groups receiving adjuvant therapy were compared with the insulin-only group (N = 26) at two time points: before the start of therapy and after 12 weeks of adjuvant therapy. All three groups were comparable regarding demographic characteristics and concomitant risk factors. Absolute and relative endothelial cell count at baseline were low and comparable to those of healthy individuals. In the group receiving empagliflozin or semaglutide, a significant increase in EPC was observed after 12 weeks of treatment. We demonstrated that EPCs have the potential to serve as markers for monitoring the efficacy of adjuvant therapy in T1DM patients. However, before their implementation in clinical practice, the flow cytometry protocol for CEC and EPC identification and quantification must be standardized. flow cytometry type 1 diabetes mellitus endothelial progenitor cells pretočna citometrija sladkorna bolezen tip 1 endotelne matične celice true false true Angleški jezik Slovenski jezik Neznano 2025-11-28 14:44:37 2025-11-28 14:44:37 2025-11-29 03:56:30 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis z dne 16. 1. 2025; str. 1-12 iss. 1, (article no. 54) Vol. 47 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 616.4 1467-3045 10.3390/cimb47010054 222632963 RAZ_Navodnik-Preloznik_Maja_2025.pdf RAZ_Navodnik-Preloznik_Maja_2025.pdf 1 5182BCBA833225E7C59ECFBDCA9B5F53 d111fb766e8c76cd14096d7ffd0abb00b20b9389d070c22ab2ff614e6a059f6c 7ad488b0-cc60-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36431 https://www.mdpi.com/1467-3045/47/1/54 1 634e1128-cc60-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36430 Univerzitetni klinični center Ljubljana 0 0 0