20.500.12556/DiRROS-24410 Expression of inducible damage-associated molecular patterns after interleukin-12 gene electrotransfer in mouse melanoma and colorectal cell lines Interleukin-12 (IL-12) has demonstrated potent antitumor effects by activating natural killer (NK) and T cells, but its systemic administration poses challenges due to toxicity. This study investigated gene electrotransfer (GET) as a localized delivery method for IL-12 plasmids in B16F10 and CT26 tumor cells and its effect on the expression of damage-associated molecular patterns (DAMPs) and several cytokines. We observed the activation of cytosolic sensors and cytokine production, with the expression of IL-6 and TNF-α upregulated only after IL-12 GET, suggesting the involvement of inducible damage-associated molecular patterns (iDAMPs) in the immune response to IL-12 gene therapy. These findings highlight the multifaceted immune activation triggered by GET, offering insights for improving IL-12-based cancer therapies. gene electrotransfer cytokine interleukin-12 elektroprenos genov citokini interlevkin-12 true false true Elsevier Masson SAS Angleški jezik Slovenski jezik by Authors Neznano 2025-11-27 03:58:38 2025-12-05 11:16:09 2025-12-06 03:38:38 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 20. 8. 2025; str. 118414-1-118414-15 no. Vol. 190 2025 0000-00-00 Zaloznikova Objavljeno apc 2025-05-20 2025-07-29 2025-08-01 602.6/.7 1950-6007 10.1016/j.biopha.2025.118414 246024963 https://doi.org/10.1016/j.biopha.2025.118414 1 f99e35f1-cb3c-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36337 Onkološki inštitut Ljubljana 0 0 0