20.500.12556/DiRROS-24383 The role of preconception parental health on embryo quality - preliminary results of a prospective study using non-invasive preimplantation genetic testing for aneuploidy In this study, we aimed to correlate embryonic ploidy status studied with non-invasive preimplantation genetic testing for aneuploidy with the basic patient characteristics of the infertile couple to gain insight into the effects of parental physical health on embryo ploidy. We recruited 131 couples, who were stratified into 4 groups based on female age. We gathered general patient characteristics of the couple and determined the female’s hormonal status. We included 316 embryos in our study. Embryos were either transferred in the uterus in a fresh cycle or vitrified for later use. We collected spent embryo culture medium on either day 5 or 6 and performed whole genome amplification before using Next Generation Sequencing. Pregnancy outcomes were noted and cross-referenced with patient characteristics and the embryo’s ploidy status in a retrospective manner. While we have indirectly observed a level of maternal contamination, we nevertheless found a significant correlation between embryo ploidy status and cell free deoxyribonucleic acid concentration in spent embryo culture, as well a correlation between female age and embryo ploidy status. We observed a significant correlation between male body mass index and cell free deoxyribonucleic acid concentration in spent embryo culture medium and between male body mass index and pregnancy outcome. We illustrated a connection between male body mass index and cell free deoxyribonucleic acid, independent of female markers. This is the first study to observe not only female but male parameters in correlation to cell free deoxyribonucleic acid. non-invasive preimplantation genetic testing aneuploidy next-generation sequencing embryo ploidy male weight female age body mass index genetsko testiranje starost ženske indeks telesne mase zarodek OBMP true false true Angleški jezik Slovenski jezik Neznano 2025-11-26 13:46:16 2025-11-26 13:46:16 2025-11-27 03:58:56 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 25. 8. 2025; str. 1-16 iss. 9, [article no.] 1215 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 618.3:575 2218-273X 10.3390/biom15091215 246450435 RAZ_Tomic_Maja_2025.pdf RAZ_Tomic_Maja_2025.pdf 1 F8DD98F0AFB4286661624B885D904BFF 906b793753fad405850bdf03f5278946cdd4701f410e11ef69d8f8d6d2ff3476 4221d453-cac6-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36300 https://doi.org/10.3390/biom15091215 0 5771a84c-cac6-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36301 Univerzitetni klinični center Ljubljana 0 0 0