20.500.12556/DiRROS-24380 Effect of tirzepatide-induced weight loss on adipose tissue in obesity rationale and design of the randomized placebo-controlled Tirzepatide Brown and Beige Adipose Tissue Activation (TABFAT) trial Background Obesity is a complex disease marked by excessive, dysfunctional adipose tissue accumulation. Recent research underscores the pivotal role of brown adipose tissue (BAT) in metabolic health and its potential as a thera- peutic target for obesity management. Emerging preclinical and clinical evidence suggests that second-generation anti-obesity drugs, especially dual agonists such as tirzepatide, may enhance BAT activity. Additionally, beige adipose tissue, derived from white adipose tissue (WAT), may contribute significantly to whole-body thermogenesis, yet its role remains underexplored. Methods This investigator-initiated, randomized, placebo-controlled clinical trial aims to evaluate the effects of tirzepatide on BAT activity and WAT browning in premenopausal women with obesity. Thirty-four participants will be randomized 1:1 to receive either tirzepatide or a placebo for 24 weeks. Primary outcomes include changes in BAT volume and activity, assessed using 18F-FDG-PET/CT, MRI, and infrared thermography, as well as the induction of WAT browning, evaluated through changes in mRNA expression patterns and histomorphometric alterations in subcuta- neous adipose tissue samples. Secondary outcomes will involve the assessment of whole-body composition, resting energy expenditure, and various metabolic health markers, correlated with thermogenic adipose tissue changes. Comparative analysis of BAT assessment methods will refine protocols for research and clinical use. Discussion This study is the first to systematically explore the potential of pharmacological obesity management to enhance BAT activity and induce WAT browning. Results may establish thermogenic adipose tissue augmentation as a novel mechanism of action for second-generation anti-obesity medications. brown adipose tissue beige adipose tissue pancreas resection tirzepatide obesity rjavo maščobno tkivo bež maščobno tkivo tirzepatid debelost true false true Angleški jezik Slovenski jezik Neznano 2025-11-26 13:42:36 2025-11-26 13:42:36 2025-11-27 03:58:56 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 24. 8. 2025; str. 1-20 issue 1, [article no.] 300 Vol. 26 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 616.4 1745-6215 10.1186/s13063-025-09045-9 246428675 RAZ_Herman_Rok_2025.pdf RAZ_Herman_Rok_2025.pdf 1 DD09720095FA8C60E680F8A5C1908DD1 d21b189681459ee0dc6b5dc258a0e931e6ba8fad3d553705b880c78b80fc2bd1 914866db-cac5-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36295 https://trialsjournal.biomedcentral.com/articles/10.1186/s13063-025-09045-9 1 66daa465-cac5-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36293 Univerzitetni klinični center Ljubljana 0 0 0