20.500.12556/DiRROS-24274 Extracellular vesicle-derived bioactive molecules for corneal and ocular surface regeneration Cell-based therapies emerge as potential treatment options for various debilitating diseases. Preclinical research and clinical studies involving cells increased exponentially in the past decade. In addition to cell-based approaches, the use of extracellular vesicles (EVs), which are released by nearly all cell types, emerged as a promising cell-free alternative. Those approaches are also being explored in the field of ophthalmology. Several clinical trials involving EVs are underway to develop potential treatments for advanced ocular surface diseases, including corneal disorders, injuries, and dry eye disease. The cargo carried by EVs has been shown to include a diverse array of functional molecules such as transcription factors, cytokines, growth factors, mRNA, tRNA, rRNA, miRNA, and fragments of dsDNA. While the molecular composition of EVs is already well characterised, the specific activity of these molecules upon delivery to recipient cells remains poorly understood. In this review, we summarise recent studies investigating the bioactive molecules within EVs shown to influence or modulate cellular activity on the ocular surface. Among these, various miRNAs have most commonly been identified as therapeutic agents targeting distinct molecular pathways. The EVs studied were predominantly derived from various mesenchymal stem cells. extracellular vesicles bioactive molecules cornea dry eye disease miRNA mesenchymal stem cells ophthalmology true false true Angleški jezik Ni določen Neznano 2025-11-24 14:23:58 2025-11-24 14:23:58 2025-11-25 04:09:24 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 11. 8. 2025; str. 1-17 iss. 15, [article no.] 5594 Vol. 14 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 617.7 2077-0383 10.3390/jcm14155594 245312259 RAZ_Kolenc_Ana_2025.pdf RAZ_Kolenc_Ana_2025.pdf 1 8723327DBA61684AE26FA8A5002A434D 8ae8751b9cfacdf09cde014d68549bc66344d65ccade08347f0fd91689f9d025 f7f42338-c938-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36163 https://www.mdpi.com/2077-0383/14/15/5594 0 0234782b-c939-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36164 Univerzitetni klinični center Ljubljana 0 0 0