20.500.12556/DiRROS-24182 Combined climate and chemical stressors how spatial variability shapes the response of Ficopomatus enigmaticus (Fauvel, 1923) to dimethyl sulfoxide (DMSO) and heatwaves, and what it means for ecotoxicology Ficopomatus enigmaticus, a reef-forming serpulid, has emerged as a promising candidate for biomonitoring and ecotoxicology studies. Recent research has focused on adult stress responses, highlighting the need to understand population-specific responses. This study employed a multi-biomarker approach to investigate how F. enigmaticus adults from two populations in the NE Adriatic (Site A) and NE Tyrrhenian (Site B) responded to chronic exposure to heat and chemical stress (dimethyl sulfoxide, DMSO), individually and in combination. The analysis detected significant differences in protein content and the activities of superoxide dismutase (SOD) and glutathione S-transferase (GST) between populations. Notably, no oxidative damage (measured as lipid peroxidation, LPO) was detected in any population or treatment. Similarly, no significant differences were detected in the integrated biomarker response index (IBRv2i). However, lower IBRv2i values at Site A suggested reduced stress conditions, possibly indicating that this site may have lower baseline stress. Overall, treatment effects were limited and site-specific: only the combined heat and DMSO exposure at Site A lowered GST activity compared to heat stress alone. Nevertheless, both populations exhibited broadly similar biochemical response patterns to stress. Our findings deepen the understanding of stress physiology in F. enigmaticus, underscoring the ecological importance of multi-stressor approaches in environmental monitoring. ecotoxicology wild populations biomarkers heat stress combined stress population responses divje populacije biomarkerji toplotni stres kombinirani stres odzivi populacij Ficopomatus enigmaticus true false true Angleški jezik Slovenski jezik Neznano 2025-11-19 11:42:02 2025-11-19 11:42:03 2025-11-20 03:44:39 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 19. 11. 2025; Soavtorji: Manuela Piccardo, Francesca Provenza, Serena Anselmi, Valentina Pitacco, Lovrenc Lipej, Stanislao Bevilacqua, Monia Renzi; str. [1]-17 iss. 6, [article no.] 181 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-08-28 2025-10-22 2025-11-01 574 2039-4713 10.3390/jox15060181 257778435 https://doi.org/10.3390/jox15060181 1 654e6dc1-c534-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36007 https://www.mdpi.com/2039-4713/15/6/181 0 92173bd3-c534-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36008 RAZ_Vellani_Verdiana_2025.pdf RAZ_Vellani_Verdiana_2025.pdf 1 0197D0895E269934337D8366782C923B 6d83dcde906372ba0bc2b2c9217098675af67243cbcef7ce4dd0a83239ec377a e6234814-c534-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36010 RAZ_Vellani_Verdiana_2025.zip RAZ_Vellani_Verdiana_2025.zip 1 4C306BFB21F89AB847CA05D797B6173D 03c8e251950167914ef997424329eec705d1402a23effd77fa2c7b49830d3bf7 cf42acb5-c534-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36009 Nacionalni inštitut za biologijo 0 0 0