20.500.12556/DiRROS-24136 Impact of infection on proteome-wide glycosylation revealed by distinct signatures for bacterial and viral pathogens Mechanisms of infection and pathogenesis have predominantly been studied based on differential gene or protein expression. Less is known about posttrans-lational modifications, which are essential for protein functional diversity. We applied an innovative glycoproteomics method to study the systemic prote-ome-wide glycosylation in response to infection. The protein site-specific glyco-sylation was characterized in plasma derived from well-defined controls and patients. We found 3862 unique features, of which we identified 463 distinct intact glycopeptides, that could be mapped to more than 30 different proteins. Statistical analyses were used to derive a glycopeptide signature that enabled significant differentiation between patients with a bacterial or viral infection. Furthermore, supported by a machine learning algorithm, we demonstrated the ability to identify the causative pathogens based on the distinctive host blood plasma glycopeptide signatures. These results illustrate that glycoproteomics holds enormous potential as an innovative approach to improve the interpreta-tion of relevant biological changes in response to infection. plasma roles glycoproteomics biomarkers profiles children glycome true false true Angleški jezik Ni določen Neznano 2025-11-17 13:27:45 2025-11-17 13:27:45 2025-11-18 03:43:32 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 22. 7. 2025; str. 1-18 iss. 8, [article no.] 107257 Vol. 26 2023 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 61 2589-0042 10.1016/j.isci.2023.107257 243429123 RAZ_Willems_Esther_2023.pdf RAZ_Willems_Esther_2023.pdf 1 742837147037264DE1210177EDE7420B 294939a689dc5e7c7c1b72a8db565de48dec7a9d62b6ded8e6e34c90873eb7f7 f63de634-c3b0-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35917 https://www.sciencedirect.com/science/article/pii/S2589004223013342 0 11dfd47e-c3b1-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35918 Univerzitetni klinični center Ljubljana 0 0 0