20.500.12556/DiRROS-24021 Correlative imaging of structural biochemistry in plant and food quality research within an interoperable data acquisition platform Correlative imaging is a powerful tool for revealing information on cell-type structures and their biochemistry, with the potential to inform healthier food choices and improved dietary recommendations. Determination of plant structures and their structural biochemistry advances our understanding of specific structures designed to store different biomolecules within cells and tissues. Compared to the classical biochemical separation techniques, the key advantage of sequential correlative imaging techniques is in relating spatial plant (micro)structures to their biochemistry in a nondestructive manner. Sequential imaging reported here comprises six methodologies on a single sample, a cross-section of a Tartary buckwheat (Fagopyrum tataricum) grain, namely, bright-field and autofluorescence microscopy, fluorescence microspectroscopy, MeV-secondary ion mass spectrometry, micro-particle-induced X-ray emission, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and laser ablation-inductively coupled plasma-mass spectrometry. Results confirm that the stepwise addition of the desired information across several classes of biomolecules and several spatial scales informs the quality and safety of plant-based produce across scales. Therefore, a viable workflow is proposed, enabling sequential spatial analysis of grain and highlighting plant structures' in situ specificity. The advantages and disadvantages of the selected methodologies were critically evaluated. autofluorescence correlative molecular imaging element distribution grain tissues avtofluorescenca molekularno slikanje razporeditev elementov true false true Angleški jezik Slovenski jezik © The Author(s) 2025. Published by Oxford University Press on behalf of the Microscopy Society of America. Neznano 2025-11-07 12:54:17 2025-11-07 14:07:28 2025-11-08 03:43:43 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Boštjan Jenčič, Martin Šala, Aleš Kladnik, Iztok Dogša, Maja Koblar, Mitja Kelemen, Primož Vavpetič, Katarina Vogel-Mikuš, Ivan Kreft, Primož Pelicon, Paula Pongrac; Opis vira z dne 28. 7. 2025; str. 1-15 iss. 4, [article no.] ozaf057 Vol. 31 Aug. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-08-01 581 1435-8115 10.1093/mam/ozaf057 243163907 https://academic.oup.com/mam/article/31/4/ozaf057/8202678 1 7eaef6c7-bbd0-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35697 RAZ_Regvar_Marjana_2025.pdf RAZ_Regvar_Marjana_2025.pdf 1 BD12317C0B8BB037C32196590E02536C 8f74abc13a270a31c6f34fe3fe3301574e83c9441b4aaba31e8f3e6a40194b41 6e786bc2-bbda-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35714 Inštitut za nutricionistiko 0 0 0