20.500.12556/DiRROS-24004 Are there clinically relevant prognostic factors in diffuse large B-cell lymphoma beyond International Prognostic Index Diffuse large B-cell lymphoma (DLBCL) has variable prognosis, with only 50 to 60% of patients cured by standard first line treatment. Identifying patients unlikely to benefit from standard first line therapy is therefore crucial. Schmitz’s study identified four molecular subtypes of DLBCL with differing prognoses: MCD, BN2, N1, and EZB, with BN2 and EZB showing more favorable outcomes. This study aimed to evaluate the effectiveness of the Archer FusionPlex Lymphoma Assay in identifying the newly defined genetic subtypes of DLBCL, while also exploring the association between immunohistochemical (IHC) and next-generation sequencing (NGS) methods for classifying the cell of origin (COO) and assessing their predictive value for patient survival. Materials and methods. We classified 131 DLBCL patients using Hans algorithm into GCB (germinal center B-celllike) and ABC (activated B-cell-like) subtypes, and with NGS applying Archer FusionPlex lymphoma assay into ABC, GCB, unclassified, and into Schmitz’s novel genetic subtypes. A mutational analysis of just 7 genes (MYD88L265P, CD79B, EZH2, NOTCH1, NOTCH2, BCL2, and BCL6) was used for genetic classification. Various statistical models were applied to assess survival differences between subtypes. Finally, STRATOS analysis was conducted to validate our preliminary statistical findings. Results. 35.9% of patients were successfully classified into new genetic subtypes, with acceptable consistency between IHC and NGS method for COO determination. However, the new genetic subtype classification by NGS did not correlate with overall survival, nor did the COO classifications by IHC or NGS. The inclusion of these classifications also did not improve the predictive value of models compared to the basic model based on the International Prognostic Index (IPI) only. Conclusions. The Archer FusionPlex Lymphoma assay showed a somewhat lower detection rate of novel genetic subtypes compared to reports based on exome sequencing, yet identified novel genetic subtypes in over one-third of patients. However, an in-depth STRATOS statistical analysis did not confirm its predictive value for DLBCL prognosis, likely due to factors like patient selection and sample size limitations. diffuse large B-cell lymphoma new genetic types prognostic factors difuzni velikocelični limfom novi genetski tipi napovedni dejavniki true false true Association of Radiology and Oncology Ljubljana Angleški jezik Slovenski jezik Neznano 2025-11-06 03:38:28 2025-11-26 10:19:10 2025-11-27 03:58:53 0000-00-00 00:00:00 2025 0 Ljubljana 0 str. [1-10] no. Vol. 2025 0000-00-00 Zaloznikova Objavljeno apc 2025-03-03 2025-03-28 2025-04-24 616.4 1318-2099 10.2478/raon-2025-0028 235496195 RAZ_Miljkovic_Milica_2025.pdf RAZ_Miljkovic_Milica_2025.pdf 1 1CFB49DD44797F35264959EC6071E105 da4057f2a8fbebdb3d1c5ec52928747a0a9b451cdbadc4404443d72267bc006d 606160de-caa9-11f0-94a7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=36265 Onkološki inštitut Ljubljana 0 0 0