20.500.12556/DiRROS-23931 Amphiphilic coumarin-based probes for live-cell STED nanoscopy of plasma membrane Plasma membranes are vital biological structures, serving as protective barriers and participating in various cellular processes. In the field of super-resolution optical microscopy, stimulated emission depletion (STED) nanoscopy has emerged as a powerful method for investigating plasma membrane-related phenomena. However, many applications of STED microscopy are critically restricted by the limited availability of suitable fluorescent probes. This paper reports on the development of two amphiphilic membrane probes, SHE-2H and SHE-2N, specially designed for STED nanoscopy. SHE-2N, in particular, demonstrates quick and stable plasma membrane labelling with negligible intracellular redistribution. Both probes exhibit outstanding photostability and resolution improvement in STED nanoscopy, and are also suited for two-photon excitation microscopy. Furthermore, microscopy experiments and cytotoxicity tests revealed no noticeable cytotoxicity of probe SHE-2N at concentration used for fluorescence imaging. Spectral analysis and fluorescence lifetime measurements conducted on probe SHE-2N using giant unilamellar vesicles, revealed that emission spectra and fluorescence lifetimes exhibited minimal sensitivity to lipid composition variations. These novel probes significantly augment the arsenal of tools available for high-resolution plasma membrane research, enabling a more profound exploration of cellular processes and dynamics fizika membrane fluorescenca true false true Elsevier Nizozemska Angleški jezik Slovenski jezik © 2024 The Authors. Neznano 2025-10-24 12:11:46 2025-10-24 12:11:46 2025-10-25 03:54:26 0000-00-00 00:00:00 2024 0 0 10 str. Art. 107554 Vol. 150 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-03-26 2024-06-09 2024-06-10 539 0045-2068 10.1016/j.bioorg.2024.107554 198949635 RAZ_Kokot_Hana_2024.pdf RAZ_Kokot_Hana_2024.pdf 1 FC1B2246D8DA82D91E7A8974EFFD6244 7867aa18bb0051938d08acecf9843d9abf1600606385f4ea4dfec72cb4e3ea72 be825b20-b0c2-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35516 Institut Jožef Stefan 0 0 0