20.500.12556/DiRROS-23715 Silane-modified lanthanide complexes for integration into hybrid coatings and nanoparticles thermal sensing applications and live cell labeling Silane-modified lanthanide complexes for integration into hybrid coatings and nanoparticles: thermal sensing applications and live cell labeling Temperature sensing at the micro- and nanoscale remains a significant challenge due to the limitations of conventional techniques in spatial resolution and invasiveness. Here, we report the development of luminescent, temperature-sensitive coatings and nanoparticles based on a newly synthesized europium (Eu) complex integrated into a composite poly(methyl methacrylate)-siloxane-silica (PMMA-siloxane silica) matrix. The Eu complex, identified as the most promising candidate among three lanthanide complexes, was functionalized with silyl ethers via copper-catalyzed azide-alkyne cycloaddition and successfully incorporated into thin PMMA-siloxane silica films on glass surfaces and within silica nanoparticles. These materials exhibited strong thermal responsiveness, chemical stability, and suitability for high-resolution temperature sensing. Furthermore, Eu-loaded silica nanoparticles showed a distinct fluorescent signal and multiplexing capability in live-cell confocal microscopy and were internalized by lung epithelial cells (LA-4 cell line), highlighting their potential as bioimaging probes for localized intracellular temperature sensing. Although some photobleaching was observed under prolonged excitation, the materials demonstrated sufficient chemical and mechanical stability, making them promising for luminescent temperature sensing and live-cell imaging. These results establish the developed Eu-based hybrid materials as robust, non-invasive luminescent probes for high-resolution thermal sensing and cellular imaging. temperature sensing silica nanoparticles europium complex photostability senzorji zaznavanja temperature nanodelci v matrici silike true false true Elsevier Nizozemska Angleški jezik Slovenski jezik © 2025 The Authors. Neznano 2025-09-26 10:48:31 2025-09-26 10:48:31 2025-10-24 03:54:55 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Soavtorji: Aljoša Bolje, Jakob Kljun, Rok Podlipec, Hana Kokot, Sebastjan Nemec, Slavko Kralj, Peter Rodič, Iztok Golobič, Stane Pajk; Opis vira z dne 25. 9. 2025; str. 1-13 Vol. 589, [article no.] 122904 Jan. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-08-25 2025-09-05 2025-09-08 621.7+621.9 1873-3255 10.1016/j.ica.2025.122904 250457603 https://www.sciencedirect.com/science/article/pii/S0020169325003706 1 960f68b8-9ab5-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35143 1-s2.0-S0020169325003706-main.pdf 1-s2.0-S0020169325003706-main.pdf 1 CDAE7348629B627B892F6898097CC2A2 6346fe66fc18f7596df284b65e674f46042ebca7555c9f780685e1c3412e2e72 8952e23b-9ab7-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35148 Institut Jožef Stefan 0 0 0