20.500.12556/DiRROS-23689 Digital PCR-based genotyping: a precision approach to HCMV drug resistance The genotyping workflow described uses digital PCR (dPCR) to detect and quantify drug resistance mutations in human cytomegalovirus (HCMV). The method focuses on the detection and quantification of three common mutations in the UL97 gene at codons 460, 594, and 595, which are responsible for the majority of ganciclovir-resistant clinical isolates. The dPCR approach offers high sensitivity and accuracy, making it suitable for routine testing as well as a reference measurement procedure for external quality assessment schemes. The workflow includes several key steps: DNA isolation, preparation of the dPCR reaction mixture, partitioning, thermocycling, and data analysis. This method improves the detection capabilities of HCMV drug resistance and provides a robust and efficient tool for clinical and research applications. digital PCR human cytomegalovirus antimicrobial drug resistance mutations CMV testi za odkrivanje mutacij virusa zunanja ocena kakovosti laboratorijska medicina true false true Angleški jezik Slovenski jezik Neznano 2025-09-25 10:16:15 2025-09-25 10:16:15 2025-10-24 03:54:51 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 22. 8. 2025; Str. 19-29 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-06-29 2026-06-29 577.2 10.1007/978-1-0716-4642-7_2 246361091 241405699 https://link.springer.com/protocol/10.1007/978-1-0716-4642-7_2 1 eaebb5a0-99e7-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35095 RAZ_Milavec_Mojca_2025.pdf RAZ_Milavec_Mojca_2025.pdf 1 F5454C140FC4A16C98C3EF95FDB7518F 69786898d8f0059e4085b2ff3a98de5df66b4bd1f627110c85a905c7bb30531d a5ebef3b-99e8-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35096 Nacionalni inštitut za biologijo 0 0 0