20.500.12556/DiRROS-23682 Evaluating plant growth–defense trade-offs by modelingthe interaction between primary and secondary metabolism Understanding the molecular mechanisms behind plant response to stress can enhancebreeding strategies and help us design crop varieties with improved stress tolerance,yield, and quality. To investigate resource redistribution from growth- to defense-relatedprocesses in an essential tuber crop, potato, here we generate a large-scale compartmen-talized genome-scale metabolic model (GEM), potato-GEM. Apart from a large-scalereconstruction of primary metabolism, the model includes the full known potato sec-ondary metabolism, spanning over 566 reactions that facilitate the biosynthesis of 182distinct potato secondary metabolites. Constraint-based modeling identifies that theactivation of the largest amount of secondary (defense) pathways occurs at a decreaseof the relative growth rate of potato leaf, due to the costs incurred by defense. We thenobtain transcriptomics data from experiments exposing potato leaves to two bioticstress scenarios, a herbivore and a viral pathogen, and apply them as constraints toproduce condition-specific models. We show that these models recapitulate experimen-tally observed decreases in relative growth rates under treatment as well as changes inmetabolite levels between treatments, enabling us to pinpoint the metabolic rewiringunderlying growth–defense trade-offs. Potato- GEM thus presents a useful resource tostudy and broaden our understanding of potato and general plant defense responsesunder stress conditions. systems biology constraint-based metabolic modeling growth-defence trade-offs secondary metabolism sistemska biologija metabolno modeliranje obramba rastlin sekundarni metabolizem true false true Angleški jezik Slovenski jezik Neznano 2025-09-24 14:03:47 2025-09-24 14:03:48 2025-10-24 03:54:50 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Sandra Correo , Maja Zagorščak, Marko Petek, Carissa Bleker, Katja Stare, Christian Schuy, Sophia Sonnewald, Kristina Gruden, Zoran Nikoloski; Opis vira z dne 20. 8. 2025; str. 1-11 no. 32, [article no.] e2502160122 Vol. 122 Aug. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-08-07 60 1091-6490 10.1073/pnas.2502160122 246042883 https://www.pnas.org/doi/10.1073/pnas.2502160122 1 88945302-993e-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35080 RAZ_Zrimec_Jan_2025.pdf RAZ_Zrimec_Jan_2025.pdf 1 32C59CADC8DAFEC0E7DFD26962732D8E 17b80730105508ae8f69cff40266b2006d01cda13b4400f9055a13f500224938 a6beab07-993e-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=35082 Nacionalni inštitut za biologijo 0 0 0