20.500.12556/DiRROS-23635 Tomato spotted wilt virus in tomato from Croatia, Montenegro and Slovenia: genetic diversity and evolution Tomato spotted wilt orthotospovirus (TSWV) is a major plant pathogen causing significant economic losses in tomato production worldwide. Understanding its genetic diversity and evolutionary mechanisms is crucial for effective disease management. This study analyzed TSWV isolates from symptomatic tomato plants collected across Croatia, Montenegro and Slovenia between 2020 and 2024. High-throughput sequencing (HTS) was employed to obtain whole-genome sequences, followed by phylogenetic analyses to assess genetic variability and relationships among isolates from these three countries and other isolates of worldwide geographic origin. Phylogenetic analyses placed all studied isolates within the L1-M3-S3 genotype, commonly associated with solanaceous crops in Europe. While Croatian and Slovenian isolates exhibited high genetic similarity, Montenegrin isolates clustered in a distinct subgroup, showing closer relationships to Asian and Mediterranean accessions. Despite the severe disease symptoms observed, no substitutions in the NSm protein associated with resistance-breaking (RB) phenotypes were detected. These findings suggest that additional virome components, environmental factors or so far unknown mechanism(s) may contribute to infection and disease severity in tomato and strongly support the need of continuous surveillance of TSWV genetic diversity in order to inform breeding programs and develop sustainable management strategies to mitigate future outbreaks. tomato HTS phylogeny TSWV plant virus paradižnik HTS filogenija TSWV rastlinski virusi true false true Angleški jezik Slovenski jezik Neznano 2025-09-16 16:02:07 2025-09-16 16:02:07 2025-10-24 03:54:43 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Jelena Zindović, Dorotea Grbin, Patrik Pul, Vladan Božović, Paolo Margaria, Nataša Mehle, Anja Pecman, Zala Kogej Zwitter, Denis Kutnjak, Ana Vučurović; Opis vira z dne 31. 7. 2025; str. 1-14 Vol. 16, [article no.] 1618327 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-07-28 632 1664-302X 10.3389/fmicb.2025.1618327 244504579 https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1618327/full 1 bda2dd67-9305-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34975 RAZ_Skoric_Dijana_2025.pdf RAZ_Skoric_Dijana_2025.pdf 1 E82FEE345D2E2974975308A81E4828C1 13182097fce3c817a096c79cc03fae94469028393637aebda5e2e5ba99920e95 fd1fb661-9306-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34976 Nacionalni inštitut za biologijo 0 0 0