20.500.12556/DiRROS-23533 Silver binding dichotomy for 7-deazaadenine/thymine preference for Watson–Crick pairing over homobase interactions in DNA DNA strands modified with 7-deazaadenine (X) and 7-deazaguanine (Y) have shown promise in forming silver-DNA assemblies while maintaining canonical Watson–Crick base pairing, highlighting the compatibility of silver binding with standard DNA structures. However, critical questions remain regarding the binding preferences of AgI ions to sequences containing 7-deazapurine bases, particularly the prevalence of silver-modified Watson–Crick base pairs versus alternative homobase pair arrangements. To address this, we examined the binding of AgI to complementary X-T sequences, demonstrating a strong preference for canonical X-AgI-T pairing over homoleptic X-AgI-X or T-AgI-T pairs. Additionally, we report the discovery of a novel metallized DNA duplex featuring continuous X-AgI-X homobase pairs, whose structural analysis at the monomeric level, using model base 9-propyl-7-deazaadenine (pX) and AgI salts, reveals a unique silver-binding pattern through the Watson–Crick face. These findings not only advance our understanding of silver-mediated DNA architectures using 7-deazapurines but also provide a foundation for the rational design of sophisticated metal-DNA nanostructures with tailored properties, opening new avenues for the development of functional DNA-based materials. crystal structure genetics ions silver solution chemistry anorganska kemija DNA nanostrukture lastnosti true false true Angleški jezik Slovenski jezik © 2025 The Authors. Published by American Chemical Society Neznano 2025-09-08 12:32:40 2025-09-08 12:49:55 2025-10-24 03:54:30 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorja iz Slovenije: Uroš Javornik, Janez Plavec; Opis vira z dne 5. 9. 2025; str. 14455–14465 iss. 28 Vol. 64 10. Jul. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-07-10 54 1520-510X 10.1021/acs.inorgchem.5c01762 247846147 https://pubs.acs.org/doi/10.1021/acs.inorgchem.5c01762?ref=pdf 1 26687929-8c9f-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34812 RAZ_Lopez-Chamorro_Carmen_2025.pdf RAZ_Lopez-Chamorro_Carmen_2025.pdf 1 9419181BBF2F69F9CB9C6D113BC538DB 026c5cf9a8c43e906f96470ff26b696216467eb98b1ad3a888be9e7386ce3ea6 4f50c0c9-8ca1-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34815 Kemijski inštitut 0 0 0