20.500.12556/DiRROS-23516 Digital PCR genotyping of pepino mosaic virus Pepino mosaic virus (PepMV) is a plant pathogen causing significant economic losses in tomato production. Sensitive, reliable, and robust detection methods are crucial for containing the spread of PepMV and reducing its damaging effects. Digital PCR (dPCR) presents several advantages to conventional real-time quantitative PCR (qPCR), including absolute quantification ability, robust quantitative multiplexing capabilities, and straightforward result analysis. Furthermore, dPCR is especially suitable for analysis of complex samples due to its remarkable tolerance to PCR inhibitors, which makes it a promising method for plant virus genotyping. In this chapter, we present two protocols for PepMV genotyping and quantification using one-step reverse transcription digital PCR (RT-dPCR). The first protocol outlines four simplex assays, while the second describes two duplex assays for precise and comprehensive genotyping of PepMV variants. plant viruses detection quantification Pepino mosaic virus PepMV digital PCR dPCR RT-dPCR duplex rastlinski virusi detekcija kvantifikacija Virus mozaika pepina (PepMV) digitalna PCR (dPCR) true false true Angleški jezik Slovenski jezik Neznano 2025-09-08 08:14:36 2025-09-08 08:14:36 2025-10-24 03:54:28 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: David Dobnik, Alexandra Bogožalec Košir, Nataša Mehle; Opis vira z dne 4. 7. 2025; Str. 31-45 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-06-29 2026-06-29 577.2 10.1007/978-1-0716-4642-7_3 241417219 241405699 https://link.springer.com/protocol/10.1007/978-1-0716-4642-7_3 1 19d236f7-8c7b-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34785 RAZ_Jelencic_Amadej_2025.pdf RAZ_Jelencic_Amadej_2025.pdf 1 4FDF08F0CFB05B6C66BE3BF479BF0DD3 b4467bc6b8da0a60b61c24ace03bc570161b5787605984532a6175b09424758b 57db0954-8c7b-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34786 Nacionalni inštitut za biologijo 0 0 0