20.500.12556/DiRROS-23502 Irrigation systems as reservoirs of diverse and pathogenic Pseudomonas syringae strains endangering crop health Pseudomonas syringae (Psy) is a widely distributed bacterial species complex primarily recognized as a foliar pathogen but also inhabits diverse environments, including water habitats, where strains closely related to agricultural pathogens have been identified. The connection between Psy-caused epidemics and its potential presence in nearby irrigation systems remains underexplored. This study comprehensively examined the Psy complex in the Danube-Tisa-Danube Hydrosystem (DTD) in Serbia, assessing its abundance, phylogenetic diversity, and pathogenic potential. To reduce the reliance on the time-consuming steps of isolation and identification, we developed novel high-specific primers and probes for precise detection of strains belonging to phylogroup 2 within Psy complex. Our results demonstrated that dPCR, coupled with highly specific and sensitive primers, outperformed both traditional plating and qPCR in detecting the Psy complex and phylogroup 2 in irrigation waters, making Psy diagnostics more effective. Phylogenetic analysis indicated high strain diversity within the DTD, identifying phylogroups 1, 2, 7, 12, and 13 and haplotypes linked to strains previously encountered in epidemics on sugar beet in Serbia. Notably, 66.67% of the isolates from the DTD were capable of inducing disease. Phylogroup 2 isolates displayed a broad host range, suggesting that the dissemination of Psy from DTD through irrigation, poses a substantial threat to crop health and agricultural productivity. phytopathogen detection irrigation qPCR dPCR detekcija patogenih bakterij namakanje qPCR dPCR true false true Angleški jezik Slovenski jezik Neznano 2025-09-05 13:44:35 2025-09-05 13:44:35 2025-10-24 03:54:27 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Iva Rosić, Olja Medić, Tamara Ranković, Karolina Sunjog, Margareta Kračun-Kolarević, Stoimir Kolarević, Tanja Dreo, Aleksander Benčič, Tanja Berić, Slaviša Stanković, Ivan Nikolić; Opis vira z dne 29. 7. 2025; str. 1-12 Vol. 28, [article no.] 100380 Sep. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-09-01 577.2 2589-9147 10.1016/j.wroa.2025.100380 244210435 https://www.sciencedirect.com/science/article/pii/S2589914725000799?via%3Dihub 1 b353337e-8a4d-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34766 RAZ_Anteljevic_Marina_2025.pdf RAZ_Anteljevic_Marina_2025.pdf 1 C683013A366D025CAFD825A908863801 452798ac70eb88869b8282c38b898632384598ebf6f9110ae057d744c2674fe6 12d3109f-8a4e-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34767 Nacionalni inštitut za biologijo 0 0 0