20.500.12556/DiRROS-23223 A comprehensive guide to potato transcriptome assembly We have witnessed a rapid advancement in high-throughput genome sequencing and the maturation of long-read technologies. However, an accurate assembly of polyploid potato genomes still remains challenging. Sequencing the double-monoploid genome of Solanum tuberosum Group Phureja (Xu et al., Nature 475:189–195, 2011) has enabled functional studies of polyploid potato cultivars using RNA sequencing (RNA-Seq) technologies, although with the limitation of not covering cultivar-specific gene expression. The accumulated RNA-Seq datasets from these cultivars can be leveraged to assemble tetraploid potato transcriptomes that enable the analysis of genes that are not limited to reference genome annotations. To increase transcriptomes’ quality, short-read assemblies are nowadays complemented with full-length transcriptome sequencing using Pacific Biosciences or Oxford Nanopore platforms. In this chapter we give a detailed guide on a pipeline for de novo transcriptome assembly of polyploid potato genotypes and their integration into a pan-transcriptome. de novo sestavljanje transkriptomov visoko zmogljive tehnologije sekveniranja pan-transkriptom FASTQ rastlinski transkriptom tetraploidni krompir RNA-sekvenciranje true false true Angleški jezik Slovenski jezik Neznano 2025-08-06 13:19:05 2025-08-06 13:19:05 2025-10-24 03:53:42 0000-00-00 00:00:00 2021 0 0 str. 155-192 2021 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-01-01 632 76788483 74599171 RAZ_Zagorscak_Maja_2021.pdf RAZ_Zagorscak_Maja_2021.pdf 1 6102649A318F94F7DA15B955901632D1 d40f1f85b7c47a409ef814dd041af11042e5b3eae18b530446e4f3d1c99b33ae a7aba522-72b7-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34189 https://link.springer.com/protocol/10.1007/978-1-0716-1609-3_8 0 b91127cd-72b7-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34190 Nacionalni inštitut za biologijo 0 0 0