20.500.12556/DiRROS-23222 Methodologies for discovery and quantitative profiling of sRNAs in potato Small RNAs (sRNAs) are short noncoding RNAs involved in the regulation of a wide range of biological processes in plants. Advances in high-throughput sequencing and development of new computational tools had facilitated the discovery of different classes of sRNAs, their quantification, and elucidation of their functional role in gene expression regulation by target transcript predictions. The workflow presented here allows identification of different sRNA species: known and novel potato miRNAs, and their sequence variants (isomiRs), as well as identification of phased small interfering RNAs (phasiRNAs). Moreover, it includes steps for differential expression analysis to search for regulated sRNAs across different tested biological conditions. In addition, it describes two different methods for predicting sRNA targets, in silico prediction, and degradome sequencing data analysis. All steps of the workflow are written in a clear and user-friendly way; thus they can be followed also by the users with minimal bioinformatics knowledge. We also included several in-house scripts together with valuable notes to facilitate data (pre)processing steps and to reduce the analysis time. rastline krompir identifikacija sekveniranje izražanje napoved tarč analiza malih RNA true false true Angleški jezik Slovenski jezik Neznano 2025-08-06 13:07:43 2025-08-06 13:07:43 2025-10-24 03:53:42 0000-00-00 00:00:00 2021 0 0 str. 221-260 2021 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2021-01-01 632 76787203 74599171 RAZ_Kriznik_Maja_2021.pdf RAZ_Kriznik_Maja_2021.pdf 1 F0DA6D3450D8B48289FF39A52B51B80A ada6606becda58841d048925fe675cc956325b352a240858cd4008596b53af83 287c3165-72b8-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34191 https://link.springer.com/protocol/10.1007/978-1-0716-1609-3_11 0 a6802ad2-72b6-11f0-9bb7-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=34188 Nacionalni inštitut za biologijo 0 0 0