20.500.12556/DiRROS-22830 Programmable protein-DNA composite nanostructures from nanostructure construction to protein-induced micro-scale material self-assembly and functionalization The integration of DNA and protein-designed nanostructures represents a transformative approach to the development of programmable biopolymers for nanoscale construction. While DNA nanostructures excel in the readily programmable precision and scalability of base pairing, protein assemblies exploit the chemical diversity of amino acids for greater functional versatility. Here a platform is presented that unifies these two paradigms by combining coiled-coil protein origami with DNA nanostructures through orthogonal protein-protein (SpyCatcher-SpyTag) and protein-DNA (DCV-DNA) covalent conjugation strategies. This dual-functionalization strategy enables the construction of stable and versatile protein-DNA composites capable of hierarchical self-assembly. This shows that these composites drive the transformation of DNA nanotubes into large-scale, patterned nanofibers or nanorods, with the proteins regularly distributed over their surface and retaining their enzymatic and fluorescent functions. In addition, a DNA-luciferase circuit is developed through split enzyme reconstitution to achieve reversible regulation of enzymatic activity, highlighting the dynamic functionality of these composites. This introduces a modular approach to producing multifunctional bio-nanomaterials, highlighting the potential of protein-DNA composite nanostructures as a bridge between molecular design and functional nanomaterials and paves the way for the development of dynamic bio-devices and programmable biomaterials. biokemija DNK beljakovine proteini nanostrukture true false true Angleški jezik Slovenski jezik © 2025 The Author(s). Small published by Wiley-VCH GmbH. Neznano 2025-07-02 07:30:47 2025-07-02 07:41:48 2025-10-24 03:52:16 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 1. 7. 2025; str. 1-12 art. 2502060 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-01-01 577 1613-6829 10.1002/smll.202502060 240986371 https://onlinelibrary.wiley.com/doi/10.1002/smll.202502060 1 b73341c2-5705-11f0-8080-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=33197 RAZ_Zhou_Weijun_2025.pdf RAZ_Zhou_Weijun_2025.pdf 1 8A93B7DF7073140AABCA16180ACD6058 7c71c929263598305c4f50d39f4cd2ae08887d940c35ea69ffe834fa83acb7de 729f7ebf-5706-11f0-8080-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=33198 Kemijski inštitut 0 0 0