20.500.12556/DiRROS-22503 Crayfish pet trade as a pathway for the introduction of known and novel viruses Expanding international pet trade has emerged as one of the main introduction pathways of aquatic invasive species, with ornamental crayfish species commonly available on the EU and global markets. Besides most frequently studied crayfish pathogens, such as Aphanomyces astaci and white spot syndrome virus (WSSV), ornamental crayfish carry associated microbial communities, which may potentially lead to the emergence of known or even novel diseases following intentional or unintentional release of animals into the wild. This is especially problematic in the case of viruses, which represent an important, yet considerably understudied, group of crayfish pathogens. Here we analyzed viromes of hepatopancreas tissue of four crayfish species acquired in the international pet trade in Europe (Procambarus clarkii, Procambarus alleni, Cherax holthuisi, and Cherax quadricarinatus) using a high throughput sequencing based metagenomic approach. Seven different known viruses were identified, which were previously either directly associated with crayfish (WSSV, Cherax quadricarinatus reovirus, chequa iflavirus, athtab bunya-like virus) or with hosts from subphylum Crustacea or invertebrates associated with freshwater environment (Shahe ispoda virus 5, Dicistroviridae sp.). Additional sequences represented 8 potential novel and divergent RNA viruses, most similar to sequences belonging to members of Picornavirales, Elliovirales, Reovirales, Hepelivirales, Tolivirales and Ghabrivirales orders. We discuss our findings in relation to their phylogenetic relationships, geographical origins, and putative pathogenicity implications. The results highlight the need for further research into the risks related to disease emergence associated with the pet trade. virome RNA viruses ornamental crayfish pet-trade high throughput sequencing ecology molecular biology pathology virology environmental risk assessment virom RNA virusi okrasni raki trgovina s hišnimi ljubljenčki visoko prepustno sekvenciranje ekologija molekularna biologija patologija virologija ocena okoljskega tveganja true false true Angleški jezik Slovenski jezik Neznano 2025-05-23 10:53:19 2025-05-23 10:53:19 2025-10-24 03:51:24 0000-00-00 00:00:00 2025 0 0 Soavtorji: Luka Kranjc, Leticia Botella, Ivana Maguire, Dora Pavić, Jiří Patoka, Paula Dragičević, Martin Bláha, Ana Bielen, Antonín Kouba, Denis Kutnjak, Sandra Hudina; str. 1-21 art. no. ǂ108345 Vol. 211 Jul. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-07-01 578 0022-2011 10.1016/j.jip.2025.108345 236953091 https://doi.org/10.1016/j.jip.2025.108345 1 61b9190d-37b3-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32516 RAZ_Bacnik_Katarina_2025.pdf RAZ_Bacnik_Katarina_2025.pdf 1 6BB450153FCDFB5133EFEB07AE0534C1 0f2cc21b5d646d0cb83e9899296d96b764f6f45d0f9cf745bd7c9e9d10514a58 942a1c0f-37b3-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32517 Supplementary_material.zip Supplementary_material.zip Dopolnilne informacije Supplementary material 1 D0380EB1A0DAB252894AB621FFE2856C 689b6fb4e16b2ac9f51d6596bb2f55b320c1519984e9dfc7e8f77c34416237aa e0888a19-37b9-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32521 Nacionalni inštitut za biologijo 0 0 0