20.500.12556/DiRROS-22224 High-resolution correlative microscopy approach for nanobio interface studies of nanoparticle-induced lung epithelial cell damage Correlated light and electron microscopy (CLEM) has become essential in life sciences due to advancements in imaging resolution, sensitivity, and sample preservation. In nanotoxicology─specifically, studying the health effects of particulate matter exposure─CLEM can enable molecular-level structural as well as functional analysis of nanoparticle interactions with lung tissue, which is key for the understanding of modes of action. In our study, we implement an integrated high-resolution fluorescence lifetime imaging microscopy (FLIM) and hyperspectral fluorescence imaging (fHSI), scanning electron microscopy (SEM), ultrahigh resolution helium ion microscopy (HIM) and synchrotron micro X-ray fluorescence (SR μXRF), to characterize the nanobio interface and to better elucidate the modes of action of lung epithelial cells response to known inflammatory titanium dioxide nanotubes (TiO2 NTs). Morpho-functional assessment uncovered several mechanisms associated with extensive DNA, essential minerals, and iron accumulation, cellular surface immobilization, and the localized formation of fibrous structures, all confirming immunomodulatory responses. These findings advance our understanding of the early cellular processes leading to inflammation development after lung epithelium exposure to these high-aspect-ratio nanoparticles. Our high-resolution experimental approach, exploiting light, ion, and electron sources, provides a robust framework for future research into nanoparticle toxicity and its impact on human health. nanobio interface nanotubes lung epithelium inflammation synchrotron micro X-ray fluorescence nanobio vmesnik nanocevke elektronska mikroskopija sinhrotronska mikro rentgenska fluorescenca true false true ACS Publications ZDA Angleški jezik Slovenski jezik © 2025 The Authors. Neznano 2025-05-12 09:53:08 2025-05-12 09:53:09 2025-10-24 03:50:51 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Opis vira z dne 12. 5. 2025; 1-17 str. no. Vol. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-12-10 2025-04-30 2025-05-09 620.3 1936-086X 10.1021/acsnano.4c17838 235464707 https://pubs.acs.org/doi/10.1021/acsnano.4c17838 1 263937c1-2f06-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32168 RAZ_Podlipec_Rok_2025.pdf RAZ_Podlipec_Rok_2025.pdf The raw data underlying this study are openly available in the HZDR RODARE repository at https://rodare.hzdr.de/record/3321 (DOI: 10.14278/rodare.3321). 1 D23BC43C2C9293DE2514FE6D85F05127 62a552f1b0940fc01aacdf1882cab1f73e358c9919da059e67fa48726dca11f9 47ecfe82-2f09-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32169 Institut Jožef Stefan 0 0 0