20.500.12556/DiRROS-22194 Nano spray-dried particles of in-situ crosslinked alginate and their toxicological characterisation The feasibility and technical capacity for producing crosslinked sub-micron gels with a nano spray-dryer were studied with variable pH systems incorporating alginate, pectin, and pullulan. The obtained powders were characterized for their morphology, particle size distribution, and their toxicological safety profile using genotoxicity and cytotoxicity assays. Additionally, quercetin was added to the encapsulation system to study the potential of the system to encapsulate this material. The produced powders exhibited morphologies and particle size distributions characteristic for nano spray-drying. The addition of pullulan and pectin to the feed solutions resulted in a particle size increase, with crosslinked alginate particles having a mean value of 1.43 μm, while particles with added pectin and pullulan had a mean particle size of 1.70 and 1.75 μm, respectively. The inclusion of quercetin proved to be problematic with this encapsulation system. Extremely high degradation rates and extremely low encapsulation efficiencies were observed due to the alkaline pH (~10) of the system that is needed to keep the feed dispersion in a liquid state and prevent premature crosslinking of the alginate. Although pectin and pullulan provided some protection for quercetin in the alkaline dispersion, the absolute quercetin content in the final product remained very low, with a maximum achieved encapsulation efficiency of 2.06 %. The safety profile of most produced powders was favourable, as they did not exhibit any significant cytotoxic and genotoxic activity in the HepG2 cell line, except in the case of Alginate/Pullulan which showed a 43 % decrease in cell viability at 500 μg/mL. Samples where quercetin was added did not show any increased toxicological effect. alginate polysaccharides crosslinking nano spray drying toxicological evaluation alginat polisaharidi zamreženje nanosušenje toksikološka ocena true false true Angleški jezik Slovenski jezik Neznano 2025-05-08 10:26:25 2025-05-08 10:26:26 2025-10-24 03:50:47 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Soavtorji: Ilja Gasan Osojnik Črnivec, Iza Rozman, Mihaela Skrt, Alja Štern, Bojana Žegura, Nataša Poklar Ulrih; Opis vira z dne 20. 11. 2024; str. 1-11 issue 2, [article no.] 137750 Vol. 283 Dec. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-11-19 577.1 1879-0003 10.1016/j.ijbiomac.2024.137750 215666435 https://www.sciencedirect.com/science/article/pii/S014181302408560X?via%3Dihub 1 244e1b82-2be6-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32118 RAZ_Levanic_Jaka_2024.pdf RAZ_Levanic_Jaka_2024.pdf 1 5FB2238DEE7F129B4A55BAE861EF3FE8 ef4ac8144d5ec5cf39e08e2764dbb7e73836aceae6a337e3426f6cb3cd9656bd 44b94038-2be7-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32119 1-s2.0-S014181302408560X-mmc1.docx 1-s2.0-S014181302408560X-mmc1.docx Dopolnilne informacije Supplementary data 1 5A7D9599978B3E07721287A29228678F 0f4cd3f36e6afa9fa7885fc79528987e443b3b6ceb6b5a03c45e9e9aefacf256 6e8d139a-2be7-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32120 Nacionalni inštitut za biologijo 0 0 0