20.500.12556/DiRROS-22192 Virome scanning of pear germplasm collections identifies a new Velarivirus and extends thegeographical spread of three other pear viruses In this study, an extensive virome investigation was performed on a germplasm collection of pear trees (Pyrus communis L.) from the Walloon Agricultural Research Centre (Gembloux, Belgium). In total,128 pear trees samples were analyzed as pools using high-throughput sequencing (HTS) techniques, and/or tested individually for targeted viruses by RT-PCR. During the virome survey, a novel velarivirus was identified in several asymptomatic trees while four known viruses were detected. Bioinformatics tools were used to assemble the genome of the new virus. The pear germplasm collection from Kozjanski Park (Slovenia) and a viral collection from Agroscope (Nyon, Switzerland) were also surveyed for the new pear virus and for three known viruses (CiVA, ARWV-1, and ARWV-2) to study their prevalence and geographic distribution. In Belgium, the new velarivirus was detected by RT-PCR in six of the 99 sampled trees (6%) and citrus virus A (CiVA) in 49 (49%) of them; in Slovenia four of the six trees sampled (67%) were positive for CiVA; and in Switzerland four of the nine trees sampled (44%) were positive for CiVA and 1 (11%) for apple rubbery wood virus 1 and 2 (ARWV-1 and -2). This study, combined pooled HTS analyses to maximize the number of germplasm tested and targeted RT-PCR tests on individual samples for accurate detection. It reports and describes a new velarivirus discovered in pear trees and first detections of CiVA in Belgium, Switzerland and Slovenia, and ARWV-1 and -2 in Switzerland. virome plant virus pear velarivirus high-throughput sequencing citrus virus A apple rubbery wood virus 1 apple rubbery wood virus 2 pyrus virus A virom rastlinski virus hruška visoko zmogljivo testiranje true false true Angleški jezik Slovenski jezik Neznano 2025-05-08 09:04:38 2025-05-08 09:04:38 2025-10-24 03:50:46 0000-00-00 00:00:00 2025 0 0 Soavtorji: Carole Gailly, Arnaud G. Blouin, Beatrix Buchmann, Markus Buenter, Thierry Candresse, Nathalie Dubuis, Denis Kutnjak, Marc Lateur, Anja Pecman, Stéphan Steyer, Sébastien Massart; Nasl. z nasl. zaslona; Opis vira z dne 12. 8. 2024; str. 68-79 no. 1 Vol. 9 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-02-12 577.2 2471-2906 10.1094/PBIOMES-03-24-0030-FI 204188675 https://apsjournals.apsnet.org/doi/10.1094/PBIOMES-03-24-0030-FI 1 b96975de-2bda-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32111 RAZ_Fontdevila_Pareta_Nuria_2025.pdf RAZ_Fontdevila_Pareta_Nuria_2025.pdf 1 FE12BE3C455B54C63681CE5A20900650 e3f568c64fd618e10b5ef91f26425df330b8d6248659da60ded72538b2ff778d e808ee8c-2bda-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32113 PBIOMES-03-24-0030-FI.sm1.zip PBIOMES-03-24-0030-FI.sm1.zip Dopolnilne informacije Supplemental Material 1 88D62BC0E14402A0CE6580ADC56D4517 55a91efa7e720538fe3fa812e7fb1eea05df8780f42d3b554da4519214b088b3 6c91d5be-2be4-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=32117 Nacionalni inštitut za biologijo 0 0 0