20.500.12556/DiRROS-22071 A long-term ecological research data set from the marine genetic monitoring program ARMS-MBON 2018–2020 Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio-temporal scales. We established an international Marine Biodiversity Observation Network (ARMS-MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard-bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open-access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to ~5500 amplicon sequence variants and ~1800 operational taxonomic units, and up to ~250 and ~50 species per observatory using the cytochrome c oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non-indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post-sequencing curation is applied to enable statistical comparison of spatio-temporal entities. We suggest that ARMS should be used in biological monitoring programs and long-term ecological research and encourage the adoption of our ARMS-MBON protocols. 18S rRNA COI essential biodiversity variables European marine omics biodiversity observation network genetic monitoring invasive species ITS 18S rRNA COI genetski monitoring ARMS Opazovalna mreža za morsko biodiverziteto (MBON) true false true Angleški jezik Slovenski jezik Neznano 2025-04-18 13:17:17 2025-04-18 13:17:18 2025-10-24 03:50:28 0000-00-00 00:00:00 2025 0 0 Soavtorji: Katrina Exter, Justine Pagnier, Piotr Balazy, Ibon Cancio, Giorgos Chatzigeorgiou, Eva Chatzinikolaou, Maciej Chelchowski, Nathan Alexis Mitchell Chrismas, Thierry Comtet, Thanos Dailianis, Klaas Deneudt, Oihane Diaz de Cerio, Markos Digenis, Vasilis Gerovasileiou, José González, Laura Kauppi, Jon Bent Kristoffersen, Piotr Kukliński, Rafał Lasota, Liraz Levy, Magdalena Małachowicz, Borut Mavrič, Jonas Mortelmans, Estefania Paredes, Anita Poćwierz-Kotus, Henning Reiss, Ioulia Santi, Georgia Sarafidou, Grigorios Skouradakis, Jostein Solbakken, Peter A. U. Staehr, Javier Tajadura, Jakob Thyrring, Jesus S. Troncoso, Emmanouela Vernadou, Frederique Viard, Haris Zafeiropoulos, Małgorzata Zbawicka, Christina Pavloudi, Matthias Obst; str. 1-19 issue ,[article no.] e14073 Vol. 25 2025 0000-00-00 PostprintKoncna VTisku NiDoloceno 0000-00-00 0000-00-00 2025-01-31 574.5 1755-098X 10.1111/1755-0998.14073 233157891 https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.14073 1 b1702fcf-1c46-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31887 RAZ_Daraghmeh_Nauras_2025.pdf RAZ_Daraghmeh_Nauras_2025.pdf 1 6840130DF8AA5989D9753E2DB2D1AE15 6e8aa5ae76f6473a7b5b3788bf863dc5de165396337978311d2a4e2640db1227 c11ceb0b-1c46-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31888 Nacionalni inštitut za biologijo 0 0 0