20.500.12556/DiRROS-22066 Diversity of the surface microbiome of canopy-forming brown macroalgae (Fucales) in the northern Adriatic Canopy-forming brown macroalgae (Fucales) offer numerous key ecosystem services in Mediterranean coastal areas. However, anthropogenic pressures and climate change have significantly impacted their habitats, leading to an extensive population decline. Interactions between algae and microbiota are a major ecological aspect, yet they represent a significant knowledge gap. In our baseline study, we describe the diversity and host specificity of the microbiome of two genetically identical but morphologically distinct populations of Gongolaria barbata from anthropogenically impacted northern Adriatic Sea. Our preliminary results showed that the microbiomes of G. barbata exhibited low host specificity, with 75% of the algae-associated amplicon sequence variants (ASVs) being part of the core coastal ecosystem microbiome. However, microbiomes of specific algal parts, ambient seawater, and sediment differed significantly in terms of alpha diversity and composition. In contrast, the holdfast and axis show higher similarity with sediment microbiomes, indicating potential horizontal transmission pathways. Microbiomes associated with deciduous parts of morphologically distinct G. barbata populations showed no difference in alpha diversity and composition. In contrast, higher variation in alpha diversity and lower sequence proportion of shared ASVs were observed in the holdfast and axis of the two distinct populations. Our observational study provides valuable new insights and baseline for future hypothesis-driven research on the interactions between algae and associated microbiota—a knowledge gap that needs to be addressed in the future for better understanding of the ecological and evolutionary dynamics of coastal ecosystems. ecosystem services anthropogenic pressures microbiome host specificity alpha diversity marine biology ecology rjave makroalge ki tvorijo krošnje sekvenciranje pomnožkov ekosistemske storitve antropogeni pritiski mikrobiom specifičnost gostitelja alfa raznovrstnost Gongolaria barbata true false true Angleški jezik Slovenski jezik Neznano 2025-04-18 10:53:31 2025-04-18 10:53:31 2025-10-24 03:50:28 0000-00-00 00:00:00 2025 0 0 Nasl. z nasl. zaslona; Soavtorji: Ana Lokovšek, Martina Orlando-Bonaca, Tinkara Tinta; Članek v PDF formatu obsega 15 str.; Online first: 16 Apr. 2025; Opis vira z dne 18. 4. 2025; [ǂart. no.] ǂe02204-24 Apr. 2025 0000-00-00 PostprintKoncna VTisku apc 0000-00-00 0000-00-00 2025-04-16 574 2165-0497 10.1128/spectrum.02204-24 233223683 https://doi.org/10.1128/spectrum.02204-24 1 9ba040b0-1c32-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31875 RAZ_Orel_Neza_2025.pdf RAZ_Orel_Neza_2025.pdf 1 69027185E1E5F9016893968EAA7F88E7 7c26c1ae79274b0523864ac80c732ec5865736f2c7413132c9041ce2ab873c16 b0864d25-1c32-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31876 Supplemental_material.zip Supplemental_material.zip Dopolnilne informacije 1 D9D3639015601EFDD0C167708BAD0F12 bf5cb1447becc9125751f67c4ef4cde376f4a58a4ea2888fd07ac456556b54c8 4b7d308a-1c39-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31882 Nacionalni inštitut za biologijo 0 0 0