20.500.12556/DiRROS-21733 Physiological and genomic characterization of two novel marine thaumarchaeal strains indicates niche differentiation Ammonia-oxidizing Archaea (AOA) are ubiquitous throughout the oceanic water column; however, our knowledge on their physiological and ecological diversity in different oceanic regions is rather limited. Here, we report the cultivation and characterization of two novel Nitrosopumilus strains, originating from coastal surface waters of the Northern Adriatic Sea. The combined physiological and genomic information revealed that each strain exhibits different metabolic and functional traits, potentially reflecting contrasting life modes. Strain NF5 contains many chemotaxis-related genes and is able to express archaella, suggesting that it can sense and actively seek favorable microenvironments such as nutrient-rich particles. In contrast, strain D3C is non-motile and shows higher versatility in substrate utilization, being able to use urea as an alternative substrate in addition to ammonia. Furthermore, it encodes a divergent, second copy of the AmoB subunit of the key enzyme ammonia monooxygenase, which might have an additional catalytic function and suggests further metabolic versatility. However, the role of this gene requires further investigation. Our results provide evidence for functional diversity and metabolic versatility among phylogenetically closely related thaumarchaeal strains, and point toward adaptations to free-living versus particle-associated life styles and possible niche differentiation among AOA in marine ecosystems. seawater ammonia oxidation bacteria picoplankton Northern Adriatic Sea genomic information Archaea Nitrosopumilus Thaumarchaeota true false true Angleški jezik Latinski jezik Neznano 2025-03-19 11:52:43 2025-03-19 11:52:43 2025-03-20 03:40:25 0000-00-00 00:00:00 2016 0 0 str. 1051-1063 Vol. 10 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2016-05-01 579 1751-7362 10.1038/ismej.2015.200 3658319 http://www.nature.com/ismej/journal/vaop/ncurrent/pdf/ismej2015200a.pdf 1 4abee6ce-04b0-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31207 RAZ_Bayer_Barbara_2016.pdf RAZ_Bayer_Barbara_2016.pdf 1 29806BF87E5A054A0FB7C0CCA916E309 cf05d6630820ab9a9d83bc0be9b6895b6bdb3bbd420363d6fb9258861be9aee3 6be41f17-04b0-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31208 http://dx.doi.org/10.1038/ismej.2015.200 0 72fa5214-04b0-11f0-963a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31209 Nacionalni inštitut za biologijo 0 0 0