20.500.12556/DiRROS-21682 Preliminary multispecies test of a model for non-lethal estimation of metabolic activity in freshwater crayfish Preliminarni test modela za oceno metabolne aktivnosti pri več vrstah potočnih rakov We tested the applicability of electron transport system (ETS) derived from a single leg as a tool for non-lethal assessment of metabolic activity in freshwater crayfish. ETS activity of the whole body and of a leg was measured in four crayfish (Arthropoda, Crustacea, Decapoda) species: two European (Astacus astacus, Austropotamobius torrentium), and two North American (Orconectes limosus, Pacifastacus leniusculus). Mass scaling of whole body ETS activity (ETSwhole) and leg ETS activity (ETSleg) was not significantly different for the European A. astacus and the American O. limosus. Therefore common models were constructed and tested on the remaining two species. The ratio ETSwhole/ETSleg was significantly positively related to body mass. In the first model (model 1) ETSwhole was calculated from ETSleg multiplied by the ratio estimated from the known body mass. ETSwhole of A. torrentium was underestimated by this model, because they mature at smaller body size than the larger species. A direct relation between ETSleg and ETSwhole was therefore proposed as a general model (model 2), since they are correlated similarly in the studied species. The results show that model 2 is suitable for estimating the whole body ETS activity from leg ETS activity for the four investigated decapods. V raziskavi smo testirali uporabnost modela za oceno metabolne aktivnosti pri potočnih rakih z merjenjem aktivnosti elektronskega transportnega sistema (ETS) le na eni nogi. Aktivnost ETS smo merili na celih osebkih in na nogi pri štirih vrstah potočnih rakov: dveh evropskih (Astacus astacus, Austropotamobius torrentium), in dveh severnoameriških (Orconectes limosus, Pacifastacus leniusculus). Ker se spreminjanje aktivnosti ETS celega telesa (ETSwhole ) in aktivnosti ETS noge (ETSleg) ni razlikovalo med jelševcem A. astacus in trnavcem O. limosus, smo naredili skupni model, ki smo ga testirali na preostalih vrstah. Razmerje ETSwhole/ETSleg je bilo v pozitivnem razmerju z maso telesa. Pri prvem modelu (model 1) smo ETSwhole izračunali iz ETSleg tako, da smo jo pomnožili z razmerjem, ki smo ga ocenili iz znane mase rakov. Izkazalo se je, da smo s tem modelom podcenili ETSwhole pri koščaku A. torrentium, verjetno zaradi nastopa zrelosti pri manjši velikosti kot pri večjih vrstah. Tako smo predlagali kot splošni model neposredno povezanost med ETSleg in ETSwhole, ki sta pri vseh vrstah rakov v podobnem razmerju. Rezultati so pokazali, da na podlagi modela 2 lahko na osnovi izmerjene aktivnosti ETS pri nogi napovemo aktivnost ETS pri vseh testiranih vrstah rakov electron transport system (ETS) activity crayfish size scaling methods (biology) aktivnost elektronskega transportnega sistema (ETS) potočni raki velikost (biologija) metode (biologija) true false true Angleški jezik Slovenski jezik Neznano 2025-03-12 10:55:04 2025-03-12 10:55:04 2025-03-13 03:52:38 0000-00-00 00:00:00 2012 0 0 Besedilo v angl.; str. 15-27 št. 1 Vol. 55 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-01 591 1408-3671 2630223 RAZ_Simcic_Tatjana_2012.pdf RAZ_Simcic_Tatjana_2012.pdf 1 3A282657411A3C974E2F1025D76E3DD4 3686a7b96b125f0c723a3b7f674fa210267c539164de9cdb452ccf52f7dbdfe2 3cf05520-ff28-11ef-88ed-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31083 http://www.dlib.si/details/URN:NBN:SI:DOC-BP8RHG1F 0 4485d955-ff28-11ef-88ed-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=31084 Nacionalni inštitut za biologijo 0 0 0