20.500.12556/DiRROS-21611 Characterization of Aureobasidium pullulans isolates selected as biocontrol agents against fruit decay pathogens The "yeast-like" fungus, Aureobasidium pullulans, isolated from fruit and leaves exhibits strong biocontrol activity against postharvest decays on various fruit. Some strains were even developed into commercial products. We obtained 20 of these strains and investigated their characteristics related to biocontrol. Phylogenetic analyses based on internal transcribed spacer (ITS) and the D1/D2 domains of rRNA 28S gene regions confirmed that all the strains are most closely related to A. pullulans species. All strains grew at 0°C, which is very important to control decay at low storage temperature, and none grew at 37°C, which eliminates concern for human safety. Eighteen strains survived 2 hrs exposures to 50°C and two strains even survived for 24 hrs. Salt-tolerance varied; however, all strains grew on medium with 14% NaCl and 14 even with 18% NaCl. Such tolerances to high temperature and elevated salinity enable compatibility with postharvest practices. Substantial differences were observed in enzymatic activity, especially with respect to production of chitinases, xylanase, or urease. Siderophore production was detected and the ability to form biofilm varied widely between the strains. Knowledge of common characteristics of these strains may be very useful in future selection of the best antagonists within this species. biological control postharvest disease management antagonism physiological variability stress tolerance biokemija biološki nadzor fiziološka spremenljivost karakterizacija izolatov true false true Angleški jezik Slovenski jezik Neznano 2025-03-04 10:41:45 2025-03-04 10:41:45 2025-03-05 03:43:59 0000-00-00 00:00:00 2020 0 0 str. 1-13 iss. 1 Vol. 10 22 Jan. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-01-22 577 2165-8056 40467461 https://www.longdom.org/open-access/characterization-of-aureobasidium-pullulans-isolates-selected-as-biocontrol-agents-against-fruit-decay-pathogens.pdf 1 e4b32a18-f8dc-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30943 RAZ_Zajc_Zunic_Janja_2020.pdf RAZ_Zajc_Zunic_Janja_2020.pdf 1 8ACB1529C708ECB76FD289ACE4329948 f74f8ad4625e060403c8adc2282cf6aad26a988590d6869cb6964889d0bb97d0 fecd215c-f8dc-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30944 Nacionalni inštitut za biologijo 0 0 0