20.500.12556/DiRROS-21608 A "crossomics" study analysing variability of different components in peripheral blood of health caucasoid individuals Background: Different immunotherapy approaches for the treatment of cancer andautoimmune diseases are being developed and tested in clinical studies worldwide. Their resulting complex experimental data should be properly evaluated, therefore reliable normal healthy control baseline values are indispensable. Methodology/Principal Findings: To assess intra- and inter-individual variability of various biomarkers, peripheral blood of 16 age and gender equilibrated healthy volunteers was sampled on 3 different days within a period of one month. Complex ććcrossomicsćć analyses of plasma metabolite profiles, antibody concentrations and lymphocyte subset counts as well as whole genome expression profiling in CD4+T and NK cells were performed. Some of the observed age, gender and BMI dependences are in agreement with the existing knowledge, like negative correlation between sex hormone levels and age or BMI related increase in lipids and soluble sugars. Thus we can assume that the distribution of all 39.743 analysed markers is well representing the normal Caucasoid population. All lymphocyte subsets, 20% of metabolites and less than 10% of genes, were identified as highly variable in our dataset. Conclusions/Significance: Our study shows that the intra-individual variability was at least two-fold lower compared to the inter-individual one at all investigated levels, showing the importance of personalised medicine approach from yet another perspective. imunoterapija protitelesa statistična analiza genska ekspresija true false true Angleški jezik Slovenski jezik Neznano 2025-03-04 10:10:12 2025-03-04 10:10:12 2025-03-05 03:43:58 0000-00-00 00:00:00 2012 0 0 Obj. online, januar 12, 2012; str. e28761 no. 1 Vol. 7 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-12 616.1 1932-6203 3192689 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0028761 1 7c180737-f8d8-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30934 RAZ_Gruden_Kristina_2012.pdf RAZ_Gruden_Kristina_2012.pdf 1 497BA1503B59E221A76BAEA6B7AA6634 71ac8f34433f8a3089081f7743a4fac3069eeb4d41f9ff42dce47f15e027c53b cdfcd7d8-f8d8-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30935 https://doi.org/10.1371/journal.pone.0028761 0 d35a5a3b-f8d8-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30936 Nacionalni inštitut za biologijo 0 0 0