20.500.12556/DiRROS-21605 Dynamics of responses in compatible potato - potato virus Y interaction are modulated by salicylic acid To investigate the dynamics of the potato – Potato virus Y (PVY) compatible interaction in relation to salicylic acid - controlled pathways we performed experiments using non-transgenic potato cv. Désirée, transgenic NahG-Désirée, cv. Igor and PVYNTN, the most aggressive strain of PVY. The importance of salicylic acid in viral multiplication and symptom development was confirmed by pronounced symptom development in NahG-Désirée, depleted in salicylic acid, and reversion of the effect after spraying with 2,6-dichloroisonicotinic acid (a salicylic acid - analogue). We have employed quantitative PCR for monitoring virus multiplication, as well as plant responses through expression of selected marker genes of photosynthetic activity, carbohydrate metabolism and the defence response. Viral multiplication was the slowest in inoculated potato of cv. Désirée, the only asymptomatic genotype in the study. The intensity of defence-related gene expression was much stronger in both sensitive genotypes (NahG-Désirée and cv. Igor) at the site of inoculation than in asymptomatic plants (cv. Désirée). Photosynthesis and carbohydrate metabolism gene expression differed between the symptomatic and asymptomatic phenotypes. The differential gene expression pattern of the two sensitive genotypes indicates that the outcome of the interaction does not rely simply on one regulatory component, but similar phenotypical features can result from distinct responses at the molecular level. plant viruses plant diseases true false true Angleški jezik Ni določen Neznano 2025-03-04 09:38:20 2025-03-04 09:38:20 2025-03-05 03:43:57 0000-00-00 00:00:00 2011 0 0 str. 1-12 issue 12 Vol. 6 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2011-12-14 578 1932-6203 10.1371/journal.pone.0029009 2492751 http://dx.doi.org/10.1371/journal.pone.0029009 1 0950002d-f8d4-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30928 RAZ_Baebler_Spela_2011.pdf RAZ_Baebler_Spela_2011.pdf 1 4B839A91FA5737452E3C23C8F75EFCC0 7b5058378717c38e922bd7e5753138ed769c6565ef0ba181a3d10b65c2d39f29 19a69aaa-f8d5-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30929 Nacionalni inštitut za biologijo 0 0 0