20.500.12556/DiRROS-21604 Characterization of GM events by insert knowledge adapted re-sequencing approaches Detection methods and data from molecular characterization of genetically modified (GM) events are needed by stakeholders of public risk assessors and regulators. Generally, the molecular characteristics of GM events are incomprehensively revealed by current approaches and biased towards detecting transformation vector derived sequences. GM events are classified based on available knowledge of the sequences of vectors and inserts (insert knowledge). Herein we present three insert knowledge-adapted approaches for characterization GM events (TT51-1 and T1c-19 rice as examples) based on paired-end re-sequencing with the advantages of comprehensiveness, accuracy and automation. The comprehensive molecular characteristics of two rice events were revealed with additional unintended insertions comparing with the results from PCR and Southern blotting. Comprehensive transgene characterization of TT51-1 and T1c-19 is shown to be independent of a priori knowledge of the insert and vector sequences employing the developed approaches. This provides an opportunity to identify and characterize also unknown GM events. molecular engineering in plants DNA recombination plant molecular biology next-generation sequencing true false true Angleški jezik Ni določen Neznano 2025-03-04 08:46:50 2025-03-04 08:46:50 2025-03-05 03:43:56 0000-00-00 00:00:00 2013 0 0 Nasl. z nasl. zaslona; Opis vira z dne 8. 10. 2013; str. 2839 [1-9] Vol. 3 2013 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2013-10-03 581.1 2045-2322 10.1038/srep02839 2924111 http://www.nature.com/srep/2013/131003/srep02839/pdf/srep02839.pdf 1 d65d6930-f8cc-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30925 RAZ_Yang_Litao_2013.pdf RAZ_Yang_Litao_2013.pdf 1 F5136730F24F7FD2458AA99E3021555F 653a30498e095962221915c5bc5c0cb3fb9df8581e00f054eb98e20bdea75d6b 3a806ebb-f8cd-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30926 https://doi.org/10.1038/srep02839 0 697073e8-f8cd-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30927 Nacionalni inštitut za biologijo 0 0 0