20.500.12556/DiRROS-21602 Clinical and structural features of sperm head vacuoles in men included in the in vitro fertilization programme The human sperm head vacuoles and their role in male infertility are still poorly understood. The aim of this study was to identify the clinical and ultrastructural features of human sperm head vacuoles in men included in the in vitro fertilization programme: men with normal (normozoospermia) and impaired sperm morphology (teratozoospermia). The sperm samples were observed under 6000-time magnification using motile sperm organelle morphology examination (MSOME). The proportion of sperm with head vacuoles was evaluated and related to the outcome of in vitro fertilization. The sperm of men with impaired sperm morphology was characterized by a higher proportion of sperm head vacuoles. The sperm head vacuoles were related to impaired semen quality (sperm concentration, motility, and morphology) but were not influenced by male factors (semen volume, height, age, weight, or body mass index). Moreover, sperm head vacuoles were related to impaired fertilization rate merely after classical in vitro fertilization (IVF), while there was no relation to pregnancy. In a subgroup of men, the sperm was fixed and observed by transmission electron microscopy (TEM). The ultrastructural study revealed that sperm head vacuoles are large nuclear indentations of various sizes and positions, packed with membranous material organized in membrane whorls (MW). human sperm head vacuoles male infertility true false true Angleški jezik Ni določen Neznano 2025-03-04 08:25:46 2025-03-04 08:25:46 2025-03-05 03:43:55 0000-00-00 00:00:00 2014 0 0 Nasl. z nasl. zaslona; Opis vira z dne 16. 4. 2014; str. 1-12 Vol. 2014 2014 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2014-04-09 576.31 2314-6141 10.1155/2014/927841 3101519 http://www.hindawi.com/journals/bmri/2014/927841/ 1 e4bd480d-f8c9-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30919 RAZ_Fekonja_Nina_2014.pdf RAZ_Fekonja_Nina_2014.pdf 1 3DB9E41226E5BEBCAA85AA56F253DCAA 6da0de5ced112fdfd6e00b07e45c4afb6adc31e5dbf59932104a6f3634f3bcdc ff9bdd99-f8c9-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30920 https://doi.org/10.1155/2014/927841 0 0a944df0-f8ca-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30921 Nacionalni inštitut za biologijo 0 0 0