20.500.12556/DiRROS-21555 The advantages of flow cytometry in comparison to fluorometric measurement in algal toxicity test Prednosti merjenja s pretočno citometrijo v primerjavi s fluorimetričnim merjenjem v strupenostnih testih z algami Fluorometric measurements in algal toxicity tests are very commonly used as surrogate parameters for algal biomass. Although, fluorometry is a powerful technique, we have demonstrated that it is not suitable for determination of toxic effects of chemicals, which alter the fluorescence spectra. We recommendthe flow cytometry as the best technique for detecting algal and/or cyanobacterial cell count and fluorescence per cell. Flow cytometry has many advantages: little volume of algal/cyanobacterial sample required, suitable also for little algal/cyanobacterial cells, distinction between live and dead cells. Furthermore, flow cytometry reveals the early changes in fluorescence spectra as a consequence of the specific chemicals presence or stress, even though the cell count is not yet affected (an early marker for ecotoxicology testing). V strupenostih testih z algami se zelo pogosto izvajajo fluorometrične meritvekot nadomestni parameter za biomaso alg. Čeprav je fluorimetrija zelo uporabna tehnika, smo pokazali, da ni primerna za določanje strupenih učinkov kemikalij, ki spremenijo spekter fluorescence. Za določanje števila alg in/alicianobakterij in njihove fluorescence na celico priporočamo uporabo pretočne citometrije. Uporaba pretočne citometrije za zaznavanje alg in/ali cianobakterij ima mnogo prednosti: majhni volumni vzorca alg/cianobakterij, primerno tudi za majhne celice alg/cianobakterij, razlikovanje med živimi in mrtvimi celicami. Poleg tega pretočna citometrija razkrije zgodnje spremembe vspektru fluorescence, ki so posledica prisotnosti specifičnih kemikalij oz. stresa, čeprav število celic še ni spremenjeno (zgodnji marker v ekotoksikoloških testiranjih). algae cyanobacteria ecotoxicity test fluorescence flow cytometry alge cianobakterije ekotoksikološki test fluorescenca pretočna citometrija true false true Angleški jezik Slovenski jezik Neznano 2025-02-26 12:58:39 2025-02-26 12:58:40 2025-02-27 03:39:02 0000-00-00 00:00:00 2012 0 0 Besedilo samo v angl.; str. 3-11 št. 2 Vol. 55 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-01 579 1408-3671 2712399 ABS_55-2_Elersek%202012.pdf ABS_55-2_Elersek%202012.pdf 1 E6D1A6770152EE5C1B96923BFF40DBF1 681226012aac06b386d884cc93d607030053221e6747a510a4657a742ce3c135 05d55714-f439-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30829 http://eprints.gozdis.si/id/eprint/14 1 1271ec12-f439-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30830 RAZ_Elersek_Tina_2012.pdf RAZ_Elersek_Tina_2012.pdf 1 8FC979527DFA9E5F48C4B2EDDFBF16B8 bf9e3add9ca3b5adcc8b71abfffd9c016ebe39b9684835d61e9d05593845b845 2e6c081f-f439-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30831 http://bijh-s.zrc-sazu.si/ABS/SI/ABS/Cont/55_2/ABS_55-2_Elersek%202012.pdf 0 4667a2d0-f439-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30832 http://www.dlib.si/details/URN:NBN:SI:DOC-LV00TE3E 0 4edc7924-f439-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30833 Nacionalni inštitut za biologijo 0 0 0