20.500.12556/DiRROS-21553 Muscle gene electrotransfer is increased by the antioxidant tempol in mice Electropermeabilization (EP) is an effective method of gene transfer into different tissues. During EP, reactive oxygen species (ROS) are formed, which could affect transfection efficiency. The role of generated ROS and the role of antioxidants in electrotransfer in myoblasts in vitro and in Musculus tibialis cranialis in mice were, therefore, investigated. We demonstrate in the study that during EP of C2C12 myoblasts, ROS are generated on the surface of the cells, which do not induce long-term genomic DNA damage. Plasmid DNA for transfection (pEGFP-N1), which is present outside the cells during EP, neutralizes the generated ROS. The ROS generation is proportional to the amplitude of the electric pulses and can be scavenged by antioxidants, such as vitamin C or tempol. When antioxidants were used during gene electrotransfer, the transfection efficiency of C2C12 myoblasts was statistically significantly increased 1.6-fold with tempol. Also in vivo, the transfection efficiency of M. tibialis cranialis in mice was statistically significantly increased 1.4-fold by tempol. The study indicates that ROS are generated on cells during EP and can be scavenged by antioxidants. Specifically, tempol can be used to improve gene electrotransfer into the muscle and possibly also to other tissues. electropermeabilization gene electrotransfer muscle tempol reactive oxygen species true false true Angleški jezik Ni določen Neznano 2025-02-26 12:40:04 2025-02-26 12:40:05 2025-02-27 03:39:01 0000-00-00 00:00:00 2012 0 0 BSDOCID163421; str. 312-320 iss. 3 Vol. 19 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2012-01-01 577.2 0969-7128 10.1038/gt.2011.97 2399567 http://dx.doi.org/10.1038/gt.2011.97 1 6ec17288-f436-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30825 RAZ_Markelc_Bostjan_2012.pdf RAZ_Markelc_Bostjan_2012.pdf 1 6CB2324EE126E92D988E1571DE97610D 0b5ab9a83e13e50ab12f4a09bacb7b9fd82a89319d95cce6421dedaa17784fa6 870c9cf3-f436-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30826 Nacionalni inštitut za biologijo 0 0 0