20.500.12556/DiRROS-21552 Modulation of the proteasome pathway by nano-curcumin and curcumin in retinal pigment epithelial cells Introduction: Curcumin has multiple biological effects including the modulation of protein homeostasis by the ubiquitin-proteasome system. The purpose of this study was to assess the in vitro cytotoxic and oxidative effects of nano-curcumin and standard curcumin and characterize their effects on proteasome regulation in retinal pigment epithelial (RPE) cells. Methods: Viability, cell cycle progression, and reactive oxygen species (ROS) production were determined after treatment with nano-curcumin or curcumin. Subsequently, the effects of nano-curcumin and curcumin on proteasome activity and the gene and protein expression of proteasome subunits PA28α, α7, β5, and β5i were assessed. Results: Nano-curcumin (5-100 μM) did not show significant cytotoxicity or anti-oxidative effects against H2O2-induced oxidative stress, whereas curcumin (≥10 μM) was cytotoxic and a potent inducer of ROS production. Both nano-curcumin and curcumin induced changes in proteasome-mediated proteolytic activity characterized by increased activity of the proteasome subunits β2 and β5i/β1 and reduced activity of β5/β1i. Likewise, nano-curcumin and curcumin affected mRNA and protein levels of household and immunoproteasome subunits. Conclusions: Nano-curcumin is less toxic to RPE cells and less prone to induce ROS production than curcumin. Both nano-curcumin and curcumin increase proteasome-mediated proteolytic activity. These results suggest that nano-curcumin may be regarded as a proteasome-modulating agent of limited cytotoxicity for RPE cells. curcumin nano-curcumin proteasome retinal pigment epithelium kurkuma nano-kurkumin proteasom true false true Angleški jezik Slovenski jezik Neznano 2025-02-26 11:49:18 2025-02-26 11:49:19 2025-02-27 03:39:01 0000-00-00 00:00:00 2018 0 0 str. 98-109 no. 2 Vol. 59 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2018-01-01 617.7 0030-3747 10.1159/000481261 4640591 RAZ_Carvalho_J._Emanuel_Ramos_de_2018.pdf RAZ_Carvalho_J._Emanuel_Ramos_de_2018.pdf 1 6596BA8506A67F002E66E6C4C9B566EE af6665dd31d3a15e53d49c063f98072542a026a873d8864194e066f44867fd3d badb23bb-f42f-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30824 https://doi.org/10.1159/000481261 0 ad11a826-f42f-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30823 Nacionalni inštitut za biologijo 0 0 0