20.500.12556/DiRROS-21528 Jellyfish blooms an overlooked hotspot and potential vector for the transmission of antimicrobial resistance in marine environments Jellyfish blooms - an overlooked hotspot and potential vector for the transmission of antimicrobial resistance in marine environments Gelatinous zooplankton (GZ) represents an important component of marine food webs, capable of generating massive blooms with severe environmental impact. When these blooms collapse, considerable amounts of organic matter (GZ-OM) either sink to the seafloor or can be introduced into the ocean’s interior, promoting bacterial growth and providing a colonizable surface for microbial interactions. We hypothesized that GZ-OM is an overlooked marine hotspot for transmitting antimicrobial resistance genes (ARGs). To test this, we first re-analyzed metagenomes from two previous studies that experimentally evolved marine microbial communities in the presence and absence of OM from Aurelia aurita and Mnemiopsis leidyi recovered from bloom events and thereafter performed additional time-resolved GZ-OM degradation experiments to improve sample size and statistical power of our analysis. We analyzed these communities for composition, ARG, and mobile genetic element (MGE) content. Communities exposed to GZ-OM displayed up to fourfold increased relative ARG and up to 10-fold increased MGE abundance per 16S rRNA gene copy compared to the controls. This pattern was consistent across ARG and MGE classes and independent of the GZ species, indicating that nutrient influx and colonizable surfaces drive these changes. Potential ARG carriers included genera containing potential pathogens raising concerns of ARG transfer to pathogenic strains. Vibrio was pinpointed as a key player associated with elevated ARGs and MGEs. Whole-genome sequencing of a Vibrio isolate revealed the genetic capability for ARG mobilization and transfer. This study establishes the first link between two emerging issues of marine coastal zones, jellyfish blooms and ARG spread, both likely increasing with future ocean change. Hence, jellyfish blooms are a quintessential “One Health” issue where decreasing environmental health directly impacts human health. gelatinous zooplankton antimicrobial resistance genes organic matter microbial interactions vibrio antimikrobna odpornost želatinozni zooplankton morski mikrobiomi horizontalni prenos genov geni za antimikrobno odpornost organske snovi mikrobne interakcije true false false Angleški jezik Slovenski jezik Neznano 2025-02-20 11:29:59 2025-02-20 11:30:00 2025-02-21 03:36:26 0000-00-00 00:00:00 2025 0 0 Soavtorji: Neža Orel, Peiju Fang, Gerhard J. Herndl, Thomas U. Berendonk, Tinkara Tinta, Uli Klümper; Nasl. z nasl. zaslona; Online first: 12 Feb. 2025; Članek v PDF formatu obsega 19 str.; Opis vira z dne 20. 2. 2025; 0000-00-00 PostprintKoncna VTisku NiDoloceno 0000-00-00 0000-00-00 2025-02-12 577 2379-5077 10.1128/msystems.01012-24 226784003 https://doi.org/10.1128/msystems.01012-24 1 a4f22a2b-ef75-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30775 RAZ_Elena_Alan_X._2025.pdf RAZ_Elena_Alan_X._2025.pdf 1 8CDB5D91B8820C24A44A054508BB9F1F 4bdeae4887d69fb3312473c050e6b31d8166c0605f08ec7a8c5347a58d6a146b 8c8fbd4e-ef76-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30776 Nacionalni inštitut za biologijo 0 0 0