20.500.12556/DiRROS-21194 Exposure to specific fungal lectins during adhesionimpairs biofilm formation of Listeria on polystyrene Listeria monocytogenes is a pathogenic bacterium that can form biofilms in food processing plants, allowing the bacteria to survive despite the control measures applied. As the surface of the bacteria is covered with versatile polysaccharides and proteins, these influence the interactions of the bacterium with any surface. The unique properties and high stability of fungal proteins make them good candidates for the control of bacteria by targeting surface structures. We screened a group of fungal lectins and protease inhibitors from different fungal species, protein folds and known targets for their antibacterial and antibiofilm activity against model strains of Listeria innocua and Listeria monocytogenes. Several of them significantly decreased the viability of biofilm bacteria, but had no effect on bacterial growth parameters at 37°C and thus had no antibacterial activity. Fungal lectins significantly impaired biofilm development even at room temperature, which was attributed to exposure to lectins during adhesion. The tested fungal proteins also reduced biofilm development on biological model surfaces. The observed antibiofilm activity of fungal proteins suggests that they have the potential to modulate interactions between bacteria and/or between bacteria and surfaces, which could be used in the future to reduce surface contamination by Listeria. Listeria monocytogenes biofilm Listeria monocytogenes biofilm Listeria monocytogenes true false true Wiley ZDA Angleški jezik Slovenski jezik © 2024 The Author(s). Neznano 2025-01-13 12:51:18 2025-01-13 12:51:19 2025-01-16 14:40:29 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Soavtorji iz Slovenije: Meta Sterniša, Blaž Jug, Tanja Zupan, Tjaša Peternel, Aleksandar Sebastijanović, Milica Perišić Nanut, Katarina Karničar, Ajda Taler-Verčič, Dušan Turk, Anja Klančnik, Janez Štrancar, Jerica Sabotič; Opis vira z dne 3. 12. 2024; str. 1-13 issue 12, [article no.] e70040 Vol. 17 Dec. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-08-20 2024-10-17 2024-11-30 577 1751-7915 10.1111/1751-7915.70040 217485315 https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.70040 1 b61f0aa3-d1a4-11ef-ba3f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30070 RAZ_Janez_Nikolaja_2024.pdf RAZ_Janez_Nikolaja_2024.pdf 1 530BAC97D583AC6A949A67BBD7D469CA b9d0eb2302dd83dc6e33eaac286b1a251920641d721e40b60035b5b38433dcb1 611d4ce6-d1a6-11ef-ba3f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30071 Institut Jožef Stefan 0 0 0