20.500.12556/DiRROS-21115 The effect of forest gaps on the diversity and composition of bacterial communities in mixed-type forest soils across the Carpathian mountains Vpliv gozdnih vrzeli na pestrost in sestavo bakterijskih združb v tleh mešanih gozdov Karpatov Mixed forests of European beech (Fagus sylvatica L.) and silver fir (Abies alba Mill.) play a vital ecological role in Central and South-Eastern Europe. This study investigates the diversity and composition of soil bacterial communities in these forests, focusing on rhizosphere and bulk soils under varying canopy structures. Soil samples were collected from eight sites along the Carpathian Mountains, including managed forests and the remnants of old growth. Metabarcoding of bacterial communities revealed that alpha diversity (species richness, Shannon index, and evenness) was significantly affected by sampling location but not by forest canopy structure or soil type (rhizosphere and bulk soil). The lowest bacterial diversity was found in the old-growth forest of the Beskidy region, while the highest was recorded in managed forest in Vrancea. Beta diversity analyses showed minimal variation between rhizosphere and bulk soil bacterial communities, with geographic distance being the strongest predictor of community composition. Actinobacteriota and Proteobacteria were the dominant phyla across all sites, with higher relative abundance of Actinobacteriota in all rhizosphere samples compared to bulk soil. Complex combinations of various environmental conditions at each sampling location, including soil parameters (mainly pH and C:N ratio), the age of forest gaps, the type and intensity of disturbances, and species composition of above-ground vegetation, can strongly affect soil bacterial communities. A closer examination of additional environmental variables would be necessary to better explain the observed differences in the diversity and composition of bacterial communities. Mešani gozdovi navadne bukve (Fagus sylvatica L.) in bele jelke (Abies alba Mill.) so eden najpomembnejših ekosistemov na območju srednje in jugovzhodne Evrope. S pristopom molekularne identifikacije smo analizirali diverziteto in sestavo talnih bakterijskih združb na osmih lokacijah vzdolž Karpatov. Metagenomska analiza bakterijskih združb je pokazala, da je na alfa diverziteto (bogastvo vrst, Shannonov indeks in enakomernost) pomembno vplivala le lokacija vzorčenja, ne pa tudi struktura gozdne krošnje ali vrsta tal (rizosfera in zemlja, oddaljena od vpliva korenin). Najmanjšo bakterijsko vrstno pestrost smo potrdili v pragozdnem rezervatu Beskidy, največjo pa v gospodarskem gozdu v Vranceji. Analiza beta diverzitete je pokazala minimalne razlike med bakterijskimi združbami rizosfere in zemlje zunaj rizosfere, pri čemer je bila geografska oddaljenost glavni dejavnik vpliva na sestavo bakterijske združbe. Actinobacteriota in Proteobacteria sta bila prevladujoča rodova bakterij na vseh analiziranih lokacijah, pri čemer je bila relativna pojavnost rodu Actinobacteriota v vseh vzorcih rizosfere višja kot v zemlji zunaj območja rizosfere. Kombinacije različnih okoljskih razmer, predvsem pH tal in razmerje med C in N, ter drugih dejavnikov, kot so starost gozdne vrzeli, intenziteta in vrsta motnje, ki je povzročila njen nastanek, ter vrstna sestava vegetacije na vzorčenih lokacijah, lahko pomembno vplivajo na mikroorganizme v tleh. Da bi bolje pojasnili razlike v pestrosti in sestavi bakterijskih združb, bi bilo treba v analize vključiti dodatne spremenljivke. forest gaps forest management soil microbiome soil rhizosphere Carpathians temperate forest gozdne vrzeli gospodarjenje z gozdom mikrobna združba tal tla rizosfera Karpati gozdovi zmernega klimatskega pasu true false true Angleški jezik Slovenski jezik Neznano 2024-12-30 10:41:11 2024-12-30 10:41:12 2025-02-12 03:35:47 0000-00-00 00:00:00 2024 0 0 str. 53-63 Vol. 135 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-01-01 630*18(045)=111 2335-3112 10.20315/ASetL.135.5 220654339 RAZ_Suban_Nejc_2024.pdf RAZ_Suban_Nejc_2024.pdf 1 2835852130439CF2878A816B916FF5FE ca785efe9bc8bddb2dd0db50b008c38a7f4debc4ee36b0dfbefa71e0f7167026 e6c96f5f-e841-11ef-9f1e-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=30649 Gozdarski inštitut Slovenije Acta Silvae et Ligni 0 0 0