20.500.12556/DiRROS-20994 Preservation of 5-hydroxymethylcytosine levels in LRIG1 across genomic DNA and cell-free DNA in glioma patients Cell-free DNA (cfDNA) has recently emerged as a promising minimally invasive diagnostic biomarker for various cancers. In this study, our aim was to identify cfDNA biomarkers by investigating genes that displayed significant differences between glioma patients and their corresponding controls. To accomplish this, we utilized publicly available data from the Gene Expression Omnibus, focusing on 5-hydroxymethylcytosine (5hmC) profiles in both cfDNA and genomic DNA (gDNA) from glioma patients and healthy individuals. The intersection of gene lists derived from these comparative analyses unveiled LRIG1 and ZNF703 as the two genes with elevated 5hmC levels in both the cfDNA of glioma patients and gDNA of glioma tissue compared to their respective controls. The gene expression data revealed both genes were upregulated in glioma tissue compared to normal brain tissue. Integration of 5hmC data revealed a strong positive correlation in the glioma tissue group between 5hmC and the gene expression of the LRIG1 gene. Furthermore, exploration using the AmiCa web tool indicated that LRIG1 gene expression was elevated compared to 17 other cancers included in the database, emphasizing its potential as a distinctive biomarker across multiple cancer types. cell-free DNA glioma 5-hydroxymethylcytosine biomarkers brezcelični DNK gliom 5-hidroksimetilcitozin biološki označevalci true false true Angleški jezik Slovenski jezik Neznano 2024-12-13 14:36:48 2024-12-13 14:36:48 2024-12-14 03:35:36 0000-00-00 00:00:00 2024 0 0 Brief report; Nasl. z nasl. zaslona; Opis vira z dne 3. 5. 2024; str. 1-8 Vol. 15 iss. 5, [article no.] 535 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-04-23 61 2073-4425 10.3390/genes15050535 194326787 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11120963/pdf/genes-15-00535.pdf 1 4eba189f-b957-11ef-ba3f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=29704 RAZ_Jevsinek_Skok_Dasa_2024.pdf RAZ_Jevsinek_Skok_Dasa_2024.pdf 1 C41D4117DD84D3E47DCDDEFAEE21890D a822fcbb353d30aa8a6902aeaa7840124d2a6ecd981e61734164f8b0bb753e95 dc17809b-b957-11ef-ba3f-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=29705 Kmetijski inštitut Slovenije 0 0 0