20.500.12556/DiRROS-20780 Impact of indoor air pollution on DNA damage and chromosome stability a systematic review Impact of indoor air pollution on DNA damage and chromosome stability: a systematic review Indoor air pollution is becoming a rising public health problem and is largely resulting from the burning of solid fuels and heating in households. Burning these fuels produces harmful compounds, such as particulate matter regarded as a major health risk, particularly affecting the onset and exacerbation of respiratory diseases. As exposure to polluted indoor air can cause DNA damage including DNA sd breaks as well as chromosomal damage, in this paper, we aim to provide an overview of the impact of indoor air pollution on DNA damage and genome stability by reviewing the scientific papers that have used the comet, micronucleus, and γ-H2AX assays. These methods are valuable tools in human biomonitoring and for studying the mechanisms of action of various pollutants, and are readily used for the assessment of primary DNA damage and genome instability induced by air pollutants by measuring different aspects of DNA and chromosomal damage. Based on our search, in selected studies (in vitro, animal models, and human biomonitoring), we found generally higher levels of DNA strand breaks and chromosomal damage due to indoor air pollutants compared to matched control or unexposed groups. In summary, our systematic review reveals the importance of the comet, micronucleus, and γ-H2AX assays as sensitive tools for the evaluation of DNA and genome damaging potential of different indoor air pollutants. Additionally, research in this particular direction is warranted since little is still known about the level of indoor air pollution in households or public buildings and its impact on genetic material. Future studies should focus on research investigating the possible impact of indoor air pollutants in complex mixtures on the genome and relate pollutants to possible health outcomes. indoor air quality genome damage comet assay micronucleus assay γ-H2AX assay health risk onesnaženje zraka zaprtih prostorov DNK poškodbe test komet test mikrojeder γ-H2AX test tveganje za zdravje true false false Angleški jezik Slovenski jezik Neznano 2024-11-06 12:11:19 2024-11-06 12:11:19 2024-11-20 08:19:50 0000-00-00 00:00:00 2024 0 0 Soavtorji: Katarina Matković, Marko Gerić, Bojana Žegura, Gordana Pehnec & Goran Gajski; str. 2817-2841 iss. 9 Vol. 98 Sept. 2024 0000-00-00 PreprintVObjavi Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-05-28 577 0340-5761 10.1007/s00204-024-03785-4 197439235 https://doi.org/10.1007/s00204-024-03785-4 1 db847fe7-9c2f-11ef-89f3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=29325 RAZ_Kazensky_Luka_2024.pdf RAZ_Kazensky_Luka_2024.pdf 1 420609F59C5C6014504C8C1C56B95E75 fbbc80963861d0fecebc34fe12f3d95782a33025093e988420287ac20955ac54 66697cd7-9c30-11ef-89f3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=29327 Nacionalni inštitut za biologijo 0 0 0