20.500.12556/DiRROS-20575 Discovery of novel and known viruses associated with toxigenic and non-toxigenic bloom forming diatoms from the Northern Adriatic Sea Algal blooms impact trophic interactions, community structure and element fluxes. Despite playing an important role in the demise of phytoplankton blooms, only few viruses infecting diatoms have been cultured. Pseudo-nitzschia is a widespread diatom genus that commonly blooms in coastal waters and contains toxin-producing species. This study describes the characterization of a novel virus infecting the toxigenic species Pseudo-nitzschia galaxiae isolated from the northern Adriatic Sea. The ssRNA virus PnGalRNAV has 29.5 nm ± 1.2 nm icosahedral virions and a genome size of 8.8 kb. It belongs to the picorna-like Marnaviridae family and shows high specificity for P. galaxiae infecting two genetically and morphologically distinct strains. We found two genetically distinct types of this virus and screening of the global virome database revealed matching sequences from the Mediterranean region and China, suggesting its global distribution. Another virus of similar shape and size infecting Pseudo-nitzschia calliantha was found, but its genome could not be determined. In addition, we have obtained and characterized a new virus that infects Chaetoceros tenuissimus. The replicase protein of this virus is very similar to the previously described ChTenDNAV type-II virus, but it has a unique genome and infection pattern. Our study is an important contribution to the collective diatom virus culture collection and will allow further investigation into how these viruses control diatom bloom termination, carbon export and toxin release in the case of Pseudo-nitzschia. algal blooms diatoms virus PnGalRNAV genome Adriatic Sea marine virology phytoplankton ecology marine biology cvetenje alg diatomeje virus genom Jadransko morje morska virologija ekologija fitoplanktona morska biologija Pseudo-nitzschia Chaetoceros Marnaviridae true false true Angleški jezik Slovenski jezik Neznano 2024-10-23 13:12:54 2024-10-23 13:12:55 2024-11-06 04:03:33 0000-00-00 00:00:00 2024 0 0 Ostali soavtorji: Denis Kutnjak, Ion Gutierrez-Aguirre, Corina P. D. Brussaard, Katarina Bačnik; Online first: 15 Oct. 2024; Članek v PDF formatu obsega 44 str.; [Art. no.] ǂ102737 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-11-01 574 1568-9883 10.1016/j.hal.2024.102737 212651523 https://doi.org/10.1016/j.hal.2024.102737 1 c2777578-912f-11ef-89f3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=29030 RAZ_Turk_Dermastia_Timotej_2024.pdf RAZ_Turk_Dermastia_Timotej_2024.pdf 1 2A4C7BD4E5DD28A34A4591CBBB6FE0FB 6d823495580723516bf2d9005ff5562b6fe1f0a0139165f59449831d1fa9dbc3 796f409a-9b60-11ef-89f3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=29306 Nacionalni inštitut za biologijo 0 0 0