20.500.12556/DiRROS-20547 Population-genetics analysis of the brown trout broodstock in the “Panjica” hatchery (Serbia) and its conservation applications Artificial propagation and stocking of brown trout is a standard practice in recreational fishery management. In recent decades, the importance of maintaining intraspecific diversity and protecting locally adapted lineages has been recognized for the species' long-term survival. The first step in selecting donors for stocking involves distinguishing native trout from non-native and introgressed individuals. The established method for discerning Atlantic hatchery strains from the wild populations involves genetic screening of individual diagnostic SNPs and microsatellite assignment tests. This study, using Serbia's Panjica hatchery as an example, illustrates the proper conduct of routine genetic screening for identifying suitable donors for supportive stocking. The broodstock and reference populations were screened using mtDNA control region, LDH nuclear gene, and 12 microsatellite loci to assess the origin, diversity, and inbreeding levels. The analysis revealed only moderate contamination with Atlantic trout and showed the regional origin of the Danubian genes – over 50% of the broodstock was composed of non-introgressed Danubian individuals tracing their origin to the Zapadna Morava River system. Additionally, the study highlighted a considerable discordance between LDH locus and microsatellites in identifying introgressed individuals, raising concerns about the sole reliance on LDH locus for the identification of Atlantic genetic origin in nuclear DNA. molecular marker discordance artificial propagation Atlantic brown trout Danubian brown trout supplementation stocking intraspecific diversity genetic screening zoology neskladja med molekulskimi označevalci vzreja postrvi postrvi atlantske linije postrvi donavske linije podporno vlaganje postrvi vrstna raznolikost genetsko presejanje zoologija true false true Angleški jezik Slovenski jezik Neznano 2024-10-21 10:24:05 2024-10-21 10:24:05 2024-10-22 03:38:16 0000-00-00 00:00:00 2024 0 0 Nasl. z nasl. zaslona; Soavtorji: Aleš Snoj, Jernej Bravničar, Vladica Simić, Radek Šanda, Jasna Vukić, Dovilė Barcytė, David Stanković, Saša Marić; Opis vira z dne 18. 10. 2024; str. 1-13 [article no.] 19 Vol. 425 Oct. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2024-10-16 639.2/.3:575 1961-9502 10.1051/kmae/2024014 212032771 https://doi.org/10.1051/kmae/2024014 1 dadfd016-8f85-11ef-89f3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28988 RAZ_Velickovic_Tijana_2024.pdf RAZ_Velickovic_Tijana_2024.pdf 1 272AF12698FCEFF186FB2B9B83F39AE6 9cb00354b61ccf1c5a3f4a0ec9f658f24b9d21fde47727f8cb80eb8a7fe45909 7c2ada41-8f86-11ef-89f3-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=28989 Nacionalni inštitut za biologijo 0 0 0